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ppar-peroxisome-proliferator–activated-receptor

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Journal Articles
Journal: Diabetes
Diabetes 2003;52(9):2331–2337
Published: 01 September 2003
...]glucose FFA, free fatty acid PPAR, peroxisome proliferator-activated receptor TZD, thiazolidinedione Increasing evidence suggests that lipid accumulation in nonadipose tissues, such as skeletal muscle and pancreatic islet, is causally related to the development of type 2 diabetes in obese...
Journal Articles
Journal: Diabetes
Diabetes 2001;50(2):411–417
Published: 01 February 2001
...Ji-Ming Ye; Patrick J. Doyle; Miguel A. Iglesias; David G. Watson; Gregory J. Cooney; Edward W. Kraegen Peroxisome proliferatoractivated receptor (PPAR)-α agonists lower circulating lipids, but the consequences for muscle lipid metabolism and insulin sensitivity are not clear. We investigated...
Journal Articles
Journal: Diabetes
Diabetes 2007;56(10):2467–2475
Published: 01 October 2007
... of this work was to determine the pattern of genes regulated by peroxisome proliferatoractivated receptor (PPAR) γ coactivator 1α (PGC-1α) in human adipocytes and the involvement of PPARα and PPARγ in PGC-1α transcriptional action. RESEARCH DESIGN AND METHODS— Primary cultures of human adipocytes were...
Includes: Supplementary data
Meeting Abstracts
Journal: Diabetes
Diabetes 1998;47(12):1841–1847
Published: 01 December 1998
... lipogenesis and triglyceride accumulation in the liver. To understand the molecular basis of the biological effects of KRP-297, we examined the effect on peroxisome proliferator-activated receptor (PPAR) isoforms, which may play key roles in lipid metabolism. Unlike classical TZD derivatives, KRP-297...
Journal Articles
Journal: Diabetes
Diabetes 2008;57(5):1394–1404
Published: 01 May 2008
...Federico Biscetti; Eleonora Gaetani; Andrea Flex; Tamar Aprahamian; Teresa Hopkins; Giuseppe Straface; Giovanni Pecorini; Egidio Stigliano; Roy C. Smith; Flavia Angelini; John J. Castellot, Jr.; Roberto Pola OBJECTIVE— Peroxisome proliferatoractivated receptors (PPARs) are therapeutic targets...
Includes: Supplementary data
Journal Articles
Journal: Diabetes
Diabetes 2005;54(12):3358–3370
Published: 01 December 2005
...Atsushi Tsuchida; Toshimasa Yamauchi; Sato Takekawa; Yusuke Hada; Yusuke Ito; Toshiyuki Maki; Takashi Kadowaki We examined the effects of activation of peroxisome proliferatoractivated receptor (PPAR)α, PPARγ, and both of them in combination in obese diabetic KKAy mice and investigated...
Journal Articles
Journal: Diabetes
Diabetes 2010;59(6):1445–1450
Published: 23 March 2010
...Dhananjay Gupta; Mina Peshavaria; Navjot Monga; Thomas L. Jetton; Jack L. Leahy OBJECTIVE We previously showed that peroxisome proliferatoractivated receptor (PPAR)-γ in β-cells regulates pdx-1 transcription through a functional PPAR response element (PPRE). Gene Bank blast...
Includes: Supplementary data
Meeting Abstracts
Journal: Diabetes
Diabetes 2021;70(Supplement_1):198-LB
Published: 01 June 2021
...PATRÍCIA V. WANDERLEY; JOAO C. DIAS; MARTA W. VIEIRA; GIANE GARCIA; ITUO T. FILHO; RAYSSA F. CHAMMA; LILIAN S. GUGONI; RAFAEL B. GIORGI; CRISTIANO R.G. BARCELLOS Background: The nuclear peroxisome proliferator-activated receptor gamma (PPAR-γ) plays a role in the regulation of glucose and lipid...
Journal Articles
Journal: Diabetes
Diabetes 2011;60(7):1990–1999
Published: 20 June 2011
... activation of STAT3, with subsequent upregulation of suppressor of cytokine signaling 3 (SOCS3). We evaluated whether peroxisome proliferatoractivated receptor (PPAR)-β/-δ prevented activation of the IL-6-STAT3-SOCS3 pathway and insulin resistance in adipocytes. RESEARCH DESIGN AND METHODS Adipocytes...
Journal Articles
Journal: Diabetes
Diabetes 2009;58(3):579–589
Published: 01 March 2009
... angiopoietin-like protein 4) represents a prominent LCFA-responsive gene in human myotubes. LCFA activated peroxisome proliferator-activated receptor (PPAR)-δ, but not PPAR-α or -γ, and pharmacological activation of PPAR-δ markedly induced ANGPTL4 production and secretion. In C2C12 myocytes, knockdown...
Includes: Supplementary data
Journal Articles
Journal: Diabetes
Diabetes 2008;57(2):332–339
Published: 01 February 2008
...; Pauline Sutton; Tim Willson; David Hassall; Keith N. Frayn; Fredrik Karpe OBJECTIVE— Pharmacological use of peroxisome proliferatoractivated receptor (PPAR)δ agonists and transgenic overexpression of PPARδ in mice suggest amelioration of features of the metabolic syndrome through enhanced fat oxidation...
Includes: Supplementary data
Images
Locations of CpG dinucleotides within the PGC1α promoter. Locations are def...
Published: 14 June 2014
Figure 1 Locations of CpG dinucleotides within the PGC1α promoter. Locations are defined by bp relative to the transcription start site ( 25 ). Putative transcription factor response elements (underlined) were identified by MatInspector ( http://www.genomatix.de/cgi-bin//matinspector ). AP2, adapt... More
Images
Liver weight, glycogen content, and gene expression analysis of Hep-M3-KO m...
Published: 14 September 2009
FIG. 3. Liver weight, glycogen content, and gene expression analysis of Hep-M3-KO mice (■) and control littermates (□) maintained on regular diet. A: Liver weight. B: Liver glycogen content of Hep-M3-KO mice and control littermates (freely fed 8-month-old males, n = 6 per group). C: Liver gene expression analysis. Gene expression was studied by real-time qRT-PCR using total hepatic RNA prepared from Hep-M3-KO mice and control littermates (freely fed 3-month-old males). Data from three independent experiments were normalized relative to the expression of cyclophilin A, which served as an internal control. Results are presented as percent change in gene expression in Hep-M3-KO mice relative to control littermates (100%). Acly, ATP citrate lyase; AOX, acyl-CoA oxidase; CPT, carnitine palmitoyltransferase; CREB, cAMP-response element binding protein; FAS, fatty acid synthase; GK, glucokinase; IR, insulin receptor; IRS1, IR substrate 1; PC, pyruvate carboxylase; PGC, PPAR coactivator; PPAR, peroxisome proliferator–activated receptor. FIG. 3. Liver weight, glycogen content, and gene expression analysis of Hep-M3-KO mice (■) and control littermates (□) maintained on regular diet. A: Liver weight. B: Liver glycogen content of Hep-M3-KO mice and control littermates (freely fed 8-month-old males, n = 6 per group). C: Liver gene expression analysis. Gene expression was studied by real-time qRT-PCR using total hepatic RNA prepared from Hep-M3-KO mice and control littermates (freely fed 3-month-old males). Data from three independent experiments were normalized relative to the expression of cyclophilin A, which served as an internal control. Results are presented as percent change in gene expression in Hep-M3-KO mice relative to control littermates (100%). Acly, ATP citrate lyase; AOX, acyl-CoA oxidase; CPT, carnitine palmitoyltransferase; CREB, cAMP-response element binding protein; FAS, fatty acid synthase; GK, glucokinase; IR, insulin receptor; IRS1, IR substrate 1; PC, pyruvate carboxylase; PGC, PPAR coactivator; PPAR, peroxisome proliferator–activated receptor. More
Images
Exercise training increases the androgen <span class="search-highlight">receptor</span> <span class="search-highlight">activity</span> in iWAT of male ...
Published: 09 February 2021
Figure 5 Exercise training increases the androgen receptor activity in iWAT of male mice. A–E: Network motif analysis by Network Analyst (A) on the most upregulated genes (FDR <0.01) with 11 days of voluntary wheel running in male iWAT (Gene Expression Omnibus data set GSE68161). List of genes regulated by AR generated by RegNetwork database (B) and relative pathway enrichment analysis using the Kyoto Encyclopedia of Genes and Genomes (KEGG) (C), GO-BP (D), and GO-CC (E) databases. F: Jaspar analysis for Ucp1 promoter region using Eukaryotic Promoter Database-Swiss Institute of Bioinformatics (SIB) website; androgen response element consensus sequences are represented as red bars. G: Quantitative chromatin immunoprecipitation PCR experiment performed on DNA samples precipitated with antibody against AR after treatment with 10 μmol/L testosterone and 10 μmol/L DHT (n = 3/group). Change in mRNA expression level for Ucp1 (H), Prkaa1 (I), Ppargc1a (J), and Esrra (K) on iWAT after 24 h of 10 mmol/L testosterone treatment (n = 6/group). Data are expressed as mean ± SEM. n.s. indicates no significant difference. *P < 0.05; ***P < 0.001. PPAR, peroxisome proliferator–activated receptor. Figure 5. Exercise training increases the androgen receptor activity in iWAT of male mice. A–E: Network motif analysis by Network Analyst (A) on the most upregulated genes (FDR <0.01) with 11 days of voluntary wheel running in male iWAT (Gene Expression Omnibus data set GSE68161). List of genes regulated by AR generated by RegNetwork database (B) and relative pathway enrichment analysis using the Kyoto Encyclopedia of Genes and Genomes (KEGG) (C), GO-BP (D), and GO-CC (E) databases. F: Jaspar analysis for Ucp1 promoter region using Eukaryotic Promoter Database-Swiss Institute of Bioinformatics (SIB) website; androgen response element consensus sequences are represented as red bars. G: Quantitative chromatin immunoprecipitation PCR experiment performed on DNA samples precipitated with antibody against AR after treatment with 10 μmol/L testosterone and 10 μmol/L DHT (n = 3/group). Change in mRNA expression level for Ucp1 (H), Prkaa1 (I), Ppargc1a (J), and Esrra (K) on iWAT after 24 h of 10 mmol/L testosterone treatment (n = 6/group). Data are expressed as mean ± SEM. n.s. indicates no significant difference. *P < 0.05; ***P < 0.001. PPAR, peroxisome proliferator–activated receptor. More
Images
Resistance to obesity in Wnt10b-<em>A</em><sup><em>y</em></sup>...
Published: 01 February 2007
FIG. 4. Resistance to obesity in Wnt10b-Ay mice at 1 year of age. Female wild-type–Ay and Wnt10b-Ay mice at 12 months of age were random fed or fasted overnight (n = 4). A: Body and adipose depot weights. d-wat, dorsolumbar white adipose tissue; O-WAT, ovarian white adipose tissue; P-WAT, perirenal white adipose tissue. B: Adipocyte area analysis and representative pictures of microscopic fields. C: Primary adipocytes were isolated from ovarian adipose tissue from wild-type–Ay and Wnt10b-Ay mice (n = 8). Gene expression was evaluated by quantitative RT-PCR for the indicated genes. Wnt10b-Ay is shown relative to wild type–Ay (means ± SE). C/EBP, CCAAT/enhancer-binding protein; FABP, fatty acid–binding protein 4; FAS, fatty acid synthase; FSP, fat-specific protein; LPL, lipoprotein lipase; PPAR, peroxisome proliferator–activated receptor. D: Blood glucose and serum proteins from random-fed (n = 4) and fasted (n = 4) mice. E: Quantitative RT-PCR of mRNA from perirenal adipose tissue comparing wild-type–Ay (n = 8) and Wnt10b-Ay (n = 8) mice. Statistical significance was evaluated using Student’s t test. *P < 0.05; **P < 0.001. FIG. 4. Resistance to obesity in Wnt10b-Ay mice at 1 year of age. Female wild-type–Ay and Wnt10b-Ay mice at 12 months of age were random fed or fasted overnight (n = 4). A: Body and adipose depot weights. d-wat, dorsolumbar white adipose tissue; O-WAT, ovarian white adipose tissue; P-WAT, perirenal white adipose tissue. B: Adipocyte area analysis and representative pictures of microscopic fields. C: Primary adipocytes were isolated from ovarian adipose tissue from wild-type–Ay and Wnt10b-Ay mice (n = 8). Gene expression was evaluated by quantitative RT-PCR for the indicated genes. Wnt10b-Ay is shown relative to wild type–Ay (means ± SE). C/EBP, CCAAT/enhancer-binding protein; FABP, fatty acid–binding protein 4; FAS, fatty acid synthase; FSP, fat-specific protein; LPL, lipoprotein lipase; PPAR, peroxisome proliferator–activated receptor. D: Blood glucose and serum proteins from random-fed (n = 4) and fasted (n = 4) mice. E: Quantitative RT-PCR of mRNA from perirenal adipose tissue comparing wild-type–Ay (n = 8) and Wnt10b-Ay (n = 8) mice. Statistical significance was evaluated using Student’s t test. *P < 0.05; **P < 0.001. More
Images
mGPDH modulates podocyte function via RAGE signaling. <em>A</em>: H...
Published: 19 March 2021
Figure 4 mGPDH modulates podocyte function via RAGE signaling. A: Heat map showing the top 30 up- and downregulated genes in isolated glomeruli from diabetic KO and WT mice. B: Gene ontology analysis of differentially expressed genes and the 10 highest-ranking biological process terms are shown. C: Top 10 ranking terms of KEGG analysis in all significantly upregulated genes. D: Immunofluorescence analysis of RAGE and the podocyte marker synaptopodin in kidneys from diabetic KO and WT mice. E: mRNA expression of genes involved in the RAGE pathway was assessed in isolated glomeruli from diabetic KO and WT mice. F: Differentiated podocytes were transfected with the mGPDH overexpression plasmid with 48 h of HG treatment, and mRNA expression of RAGE pathway-related genes is shown. GP: Podocytes were knocked down with mGPDH and/or RAGE with their specific siRNA and treated with HG for 48 h. DNA fragmentation (G), immunofluorescence staining of the indicated podocyte proteins (H), mRNA expression of the indicated genes (I), OCR and ECAR (J), mtDNA content (K), MitoTracker Green fluorescence (L), MMP (M), mRNA of OXPHOS genes (N), and Ppargc1a (O) and ROS generation (P) detected by the median fluorescence intensity (MFI) of DCF fluorescence are shown. Scale bars: 20 μm for D and H. n = 3 for A–C and F–P; n = 6 mice/group for D and E. The data are presented as means ± SEM. *P < 0.05; **P < 0.01; ***P < 0.001. PPAR, peroxisome proliferator–activated receptor; Si-ctrl, siRNA control; TNF-α, tumor necrosis factor-α. Figure 4. mGPDH modulates podocyte function via RAGE signaling. A: Heat map showing the top 30 up- and downregulated genes in isolated glomeruli from diabetic KO and WT mice. B: Gene ontology analysis of differentially expressed genes and the 10 highest-ranking biological process terms are shown. C: Top 10 ranking terms of KEGG analysis in all significantly upregulated genes. D: Immunofluorescence analysis of RAGE and the podocyte marker synaptopodin in kidneys from diabetic KO and WT mice. E: mRNA expression of genes involved in the RAGE pathway was assessed in isolated glomeruli from diabetic KO and WT mice. F: Differentiated podocytes were transfected with the mGPDH overexpression plasmid with 48 h of HG treatment, and mRNA expression of RAGE pathway-related genes is shown. G–P: Podocytes were knocked down with mGPDH and/or RAGE with their specific siRNA and treated with HG for 48 h. DNA fragmentation (G), immunofluorescence staining of the indicated podocyte proteins (H), mRNA expression of the indicated genes (I), OCR and ECAR (J), mtDNA content (K), MitoTracker Green fluorescence (L), MMP (M), mRNA of OXPHOS genes (N), and Ppargc1a (O) and ROS generation (P) detected by the median fluorescence intensity (MFI) of DCF fluorescence are shown. Scale bars: 20 μm for D and H. n = 3 for A–C and F–P; n = 6 mice/group for D and E. The data are presented as means ± SEM. *P < 0.05; **P < 0.01; ***P < 0.001. PPAR, peroxisome proliferator–activated receptor; Si-ctrl, siRNA control; TNF-α, tumor necrosis factor-α. More
Meeting Abstracts
Journal: Diabetes
Diabetes 1999;48(7):1466–1468
Published: 01 July 1999
...F P Mancini; O Vaccaro; L Sabatino; A Tufano; A A Rivellese; G Riccardi; V Colantuoni Peroxisome proliferator-activated receptor (PPAR)-gamma is a major regulator of adipogenesis and insulin sensitivity. The PPAR-gamma gene generates two isoforms through alternative splicing, PPAR-gamma1...
Images
Proposed novel cross talk between the PVAT and vascular wall. 4-HNE, 4-hydr...
Published: 16 May 2015
Figure 1 Proposed novel cross talk between the PVAT and vascular wall. 4-HNE, 4-hydroxynonenal; PI3K/Akt, phosphoinositide-3 kinase/protein kinase B; PPAR-γ, peroxisome proliferator–activated receptor-γ. Figure 1. Proposed novel cross talk between the PVAT and vascular wall. 4-HNE, 4-hydroxynone... More
Journal Articles
Journal: Diabetes
Diabetes 2002;51(3):867–870
Published: 01 March 2002
... expression are not completely understood. Peroxisome proliferatoractivated receptor (PPAR)-γ is a nuclear receptor regulating lipid and glucose metabolism, and a PPAR-responsive element is present in the LPL promoter. We determined the Pro12Ala polymorphism in the PPAR-γ2 gene in 194 male CAD patients...
Journal Articles
Journal: Diabetes
Diabetes 2004;53(suppl_1):S66–S70
Published: 01 February 2004
...So-youn Kim; Ha-il Kim; Sang-Kyu Park; Seung-Soon Im; Tianzhu Li; Hyae Gyeong Cheon; Yong-ho Ahn Thiazolidinediones (TZDs), synthetic ligands of peroxisome proliferator-activated receptor (PPAR)-γ, are known to decrease hepatic glucose production and increase glycogen synthesis in diabetic animals...