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TABLE 1

Detection of T-cell responses

AssayResult
Laboratory no. 1  
 Proliferation Responses to GAD(524-543) and insulin (9-23) detected (SIs ∼10) 
Laboratory no. 2  
 Proliferation No responses detected 
Laboratory no. 3  
 Proliferation No responses to GAD(524-543) or insulin(9-23) Responses detected to GAD(217-236) (average SI ∼3) 
 ELISA analysis of cytokines in culture supernatants Responses detected to GAD(524-543) and insulin(9-23)(∼5-fold over background) and to GAD(217-236) (∼43-fold over background) 
Laboratory no. 4  
 Proliferation No responses detected 
Laboratory no. 5  
 Proliferation No responses detected 
 Preexpansion of cells followed by ELISPOT Responses detected to GAD(524-543) (>100 spots over background) but not to insulin(9-23) 
Laboratory no. 6  
 Proliferation No responses detected 
Laboratory no. 7  
 Proliferation No responses detected 
Laboratory no. 8  
 Proliferation No responses detected 
Laboratory no. 10  
 Proliferation No responses detected 
 ELISPOT No responses detected 
Laboratory no. 12  
 Proliferation No responses detected 
 CFSE-FACS No responses detected 
 ELISPOT No responses detected 
Laboratory no. 13  
 Proliferation No responses detected 
AssayResult
Laboratory no. 1  
 Proliferation Responses to GAD(524-543) and insulin (9-23) detected (SIs ∼10) 
Laboratory no. 2  
 Proliferation No responses detected 
Laboratory no. 3  
 Proliferation No responses to GAD(524-543) or insulin(9-23) Responses detected to GAD(217-236) (average SI ∼3) 
 ELISA analysis of cytokines in culture supernatants Responses detected to GAD(524-543) and insulin(9-23)(∼5-fold over background) and to GAD(217-236) (∼43-fold over background) 
Laboratory no. 4  
 Proliferation No responses detected 
Laboratory no. 5  
 Proliferation No responses detected 
 Preexpansion of cells followed by ELISPOT Responses detected to GAD(524-543) (>100 spots over background) but not to insulin(9-23) 
Laboratory no. 6  
 Proliferation No responses detected 
Laboratory no. 7  
 Proliferation No responses detected 
Laboratory no. 8  
 Proliferation No responses detected 
Laboratory no. 10  
 Proliferation No responses detected 
 ELISPOT No responses detected 
Laboratory no. 12  
 Proliferation No responses detected 
 CFSE-FACS No responses detected 
 ELISPOT No responses detected 
Laboratory no. 13  
 Proliferation No responses detected 

FACS, –fluorescence activated cell sorter.

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