Primers used for quantitation of mRNA by real-time qPCR
| Gene . | Accession no. . | 5’ primer (5’–3’) . | 3’ primer (5’–3’) . |
|---|---|---|---|
| aP2 | NM_053365 | ATGTGTGATGCCTTTGTGGG | CCCAGTTTGAAGGAAATCTC |
| Acadl | NM_012819 | AGCTCCCACAGGAAAGGCTT | CTCGAGCATCCACGTAGGCT |
| ATGL | XM_341960 | CACTTTAGCTCCAAGGATGA | TGGTTCAGTAGGCCATTCCT |
| DGAT-1 | NM_053437 | TATTACTTCATCTTTGCTCC | AAAGTAGGTGACAGACTCAG |
| GyK | NM_024381 | CCTGTCCATTGAAATGTGTCATCC | GCCATGAAGCCATGACAATTAGTG |
| LPL | NM_012598 | AACCTTTGTGGTGATCCATGGA | CGAAATCCGCATCATCAGGA |
| L27 | NM_022514 | CTGCTCGCTGTCGAAATG | CCTTGCGTTTCAGTGCTG |
| mCPT-1 | NM_013200 | CGGAAGCACACCAGGCAGTA | GCAGCTTCAGGGTTTGTCGGAATA |
| PDK-2 | NM_030872 | GGGGTGTCCCCTTGAGGAAGAT | TTCTTGGGCTCTGTGCTGGG |
| PDK-4 | NM_053551 | TTCTACTCGGATGCTCATGA | CACCTCGGTCAGAAATCTTG |
| PEPCK | NM_198780 | TGGGTGATGACATTGCCTGG | ACCTTGCCCTTATGCTCTGCAG |
| PGC-1α | NM_031347 | TCCTGTTACTATTATGAATCAAGCC | AAACCATAGCTGTCTCCATCATCC |
| PPARγ 2 | AF156666 | GGTGAAACTCTGGGAGATCC | TGAGGGAGTTTGAAGACTCTTC |
| UCP-1 | NM_012682 | TGGTGAGTTCGACAACTTCC | GTGGGCTGCCCAATGAATAC |
| Gene . | Accession no. . | 5’ primer (5’–3’) . | 3’ primer (5’–3’) . |
|---|---|---|---|
| aP2 | NM_053365 | ATGTGTGATGCCTTTGTGGG | CCCAGTTTGAAGGAAATCTC |
| Acadl | NM_012819 | AGCTCCCACAGGAAAGGCTT | CTCGAGCATCCACGTAGGCT |
| ATGL | XM_341960 | CACTTTAGCTCCAAGGATGA | TGGTTCAGTAGGCCATTCCT |
| DGAT-1 | NM_053437 | TATTACTTCATCTTTGCTCC | AAAGTAGGTGACAGACTCAG |
| GyK | NM_024381 | CCTGTCCATTGAAATGTGTCATCC | GCCATGAAGCCATGACAATTAGTG |
| LPL | NM_012598 | AACCTTTGTGGTGATCCATGGA | CGAAATCCGCATCATCAGGA |
| L27 | NM_022514 | CTGCTCGCTGTCGAAATG | CCTTGCGTTTCAGTGCTG |
| mCPT-1 | NM_013200 | CGGAAGCACACCAGGCAGTA | GCAGCTTCAGGGTTTGTCGGAATA |
| PDK-2 | NM_030872 | GGGGTGTCCCCTTGAGGAAGAT | TTCTTGGGCTCTGTGCTGGG |
| PDK-4 | NM_053551 | TTCTACTCGGATGCTCATGA | CACCTCGGTCAGAAATCTTG |
| PEPCK | NM_198780 | TGGGTGATGACATTGCCTGG | ACCTTGCCCTTATGCTCTGCAG |
| PGC-1α | NM_031347 | TCCTGTTACTATTATGAATCAAGCC | AAACCATAGCTGTCTCCATCATCC |
| PPARγ 2 | AF156666 | GGTGAAACTCTGGGAGATCC | TGAGGGAGTTTGAAGACTCTTC |
| UCP-1 | NM_012682 | TGGTGAGTTCGACAACTTCC | GTGGGCTGCCCAATGAATAC |