Effect of long-term high-fat feeding in wild-type and PPAR-α–null mice on two genetic backgrounds
| . | C57BL/6N mice . | Sv/129 mice . | ||||||
|---|---|---|---|---|---|---|---|---|
| Wild-type . | PPAR-α–null . | Wild-type . | PPAR-α–null . | |||||
| Standard diet (n = 6) . | High-fat diet (n = 6) . | Standard diet (n = 6) . | High-fat diet (n = 6) . | Standard diet (n = 8) . | High-fat diet (n = 8) . | Standard diet (n = 8) . | High-fat diet (n = 8) . | |
| Food intake (kcal · kg−1 · day−1) | 465 ± 28.5 | 511 ± 15.2 | 506 ± 21.0 | 560 ± 28.9 | 383 ± 7.35 | 414 ± 13.3 | 369 ± 11.6 | 386 ± 22.4 |
| Body weight (g) | 32.7 ± 0.95 | 38.0 ± 1.37† | 30.8 ± 0.94 | 32.4 ± 0.78 | 27.1 ± 0.80 | 34.5 ± 1.28† | 29.6 ± 0.70 | 33.1 ± 0.90 |
| Liver weight (g) | 1.46 ± 0.12 | 1.44 ± 0.10 | 1.50 ± 0.07 | 1.48 ± 0.11 | 1.00 ± 0.03 | 1.26 ± 0.05† | 1.26 ± 0.08 | 1.95 ± 0.10† |
| Adiposity index* (%) | 1.60 ± 0.33 | 3.58 ± 0.34† | 1.71 ± 0.25 | 3.74 ± 0.40† | 2.94 ± 0.23 | 6.34 ± 0.78† | 3.42 ± 0.36 | 5.93 ± 0.52† |
| Plasma glucose (mmol/l) | 9.8 ± 0.67 | 11.5 ± 0.75 | 10.6 ± 0.72 | 9.3 ± 0.42 | 9.6 ± 0.56 | 11.6 ± 0.58 | 9.1 ± 0.41 | 8.2 ± 0.65 |
| Plasma insulin (pmol/l) | 209 ± 21.3 | 398 ± 50.1† | 189 ± 31.8 | 225 ± 30.2 | 215 ± 23.9 | 378 ± 38.8† | 284 ± 26.9 | 256 ± 32.3 |
| . | C57BL/6N mice . | Sv/129 mice . | ||||||
|---|---|---|---|---|---|---|---|---|
| Wild-type . | PPAR-α–null . | Wild-type . | PPAR-α–null . | |||||
| Standard diet (n = 6) . | High-fat diet (n = 6) . | Standard diet (n = 6) . | High-fat diet (n = 6) . | Standard diet (n = 8) . | High-fat diet (n = 8) . | Standard diet (n = 8) . | High-fat diet (n = 8) . | |
| Food intake (kcal · kg−1 · day−1) | 465 ± 28.5 | 511 ± 15.2 | 506 ± 21.0 | 560 ± 28.9 | 383 ± 7.35 | 414 ± 13.3 | 369 ± 11.6 | 386 ± 22.4 |
| Body weight (g) | 32.7 ± 0.95 | 38.0 ± 1.37† | 30.8 ± 0.94 | 32.4 ± 0.78 | 27.1 ± 0.80 | 34.5 ± 1.28† | 29.6 ± 0.70 | 33.1 ± 0.90 |
| Liver weight (g) | 1.46 ± 0.12 | 1.44 ± 0.10 | 1.50 ± 0.07 | 1.48 ± 0.11 | 1.00 ± 0.03 | 1.26 ± 0.05† | 1.26 ± 0.08 | 1.95 ± 0.10† |
| Adiposity index* (%) | 1.60 ± 0.33 | 3.58 ± 0.34† | 1.71 ± 0.25 | 3.74 ± 0.40† | 2.94 ± 0.23 | 6.34 ± 0.78† | 3.42 ± 0.36 | 5.93 ± 0.52† |
| Plasma glucose (mmol/l) | 9.8 ± 0.67 | 11.5 ± 0.75 | 10.6 ± 0.72 | 9.3 ± 0.42 | 9.6 ± 0.56 | 11.6 ± 0.58 | 9.1 ± 0.41 | 8.2 ± 0.65 |
| Plasma insulin (pmol/l) | 209 ± 21.3 | 398 ± 50.1† | 189 ± 31.8 | 225 ± 30.2 | 215 ± 23.9 | 378 ± 38.8† | 284 ± 26.9 | 256 ± 32.3 |
Data are means ± SE. Mice on the C 57BL/6N genetic background were fed the high-fat diet for 16 weeks. Mice on the Sv/129 genetic background were fed the high-fat diet for 22 weeks.
Adiposity index was the ratio of epididymal adipose tissue to body weight for C57BL/6N mice and the ratio of epididymal + inguinal adipose tissue to body weight for Sv/129 mice.
P < 0.05 versus standard diet.