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TABLE 1

Cytotoxic effect of oleate and palmitate in cultured rat β-cell preparations

Exposure time (mmol/l)Cytotoxicity
2 days8 days
Oleate 0.125 <5 7 ± 1* 
 0.250 7 ± 2* 13 ± 2 
 0.500 9 ± 2* 25 ± 2 
Palmitate 0.125 5 ± 1* 15 ± 2 
 0.250 12 ± 2 43 ± 3 
 0.500 54 ± 5 94 ± 2 
Oleate 0.125 <5 <5§ 
Palmitate 0.125   
Oleate 0.250 7 ± 1 13 ± 3 
Palmitate 0.250   
Bromopalmitate 0.125 5 ± 1# 37 ± 3 
 0.250 13 ± 2 77 ± 1 
 0.500 75 ± 6 100 ± 0 
Exposure time (mmol/l)Cytotoxicity
2 days8 days
Oleate 0.125 <5 7 ± 1* 
 0.250 7 ± 2* 13 ± 2 
 0.500 9 ± 2* 25 ± 2 
Palmitate 0.125 5 ± 1* 15 ± 2 
 0.250 12 ± 2 43 ± 3 
 0.500 54 ± 5 94 ± 2 
Oleate 0.125 <5 <5§ 
Palmitate 0.125   
Oleate 0.250 7 ± 1 13 ± 3 
Palmitate 0.250   
Bromopalmitate 0.125 5 ± 1# 37 ± 3 
 0.250 13 ± 2 77 ± 1 
 0.500 75 ± 6 100 ± 0 

Data are means ± SE for six to nine experiments. FFA-induced toxicity after 2 and 8 days of culture at 10 mmol/l glucose and 1% bovine serum albumin. Cytotoxicity of single fatty acids was assessed by comparison with control condition (no fatty acids added):

*

P < 0.01;

P < 0.001;

P < 0.01 vs. condition without palmitate;

§

P < 0.01;

P < 0.05;

P < 0.001 vs. condition without oleate;

#

P < 0.05. (analysis of variance, followed by paired t test with Bonferroni correction).

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