American Diabetes Association

TABLE 1

Cytotoxic effect of oleate and palmitate in cultured rat β-cell preparations

	Exposure time (mmol/l)	Cytotoxicity
	Exposure time (mmol/l)	2 days	8 days
Oleate	0.125	<5	7 ± 1^*
	0.250	7 ± 2^*	13 ± 2^†
	0.500	9 ± 2^*	25 ± 2^†
Palmitate	0.125	5 ± 1^*	15 ± 2^†
	0.250	12 ± 2^†	43 ± 3^†
	0.500	54 ± 5^†	94 ± 2^†
Oleate	0.125	<5	<5^‡^§
Palmitate	0.125
Oleate	0.250	7 ± 1^∥	13 ± 3^¶
Palmitate	0.250
Bromopalmitate	0.125	5 ± 1^#	37 ± 3^†
	0.250	13 ± 2^†	77 ± 1^†
	0.500	75 ± 6^†	100 ± 0^†

	Exposure time (mmol/l)	Cytotoxicity
	Exposure time (mmol/l)	2 days	8 days
Oleate	0.125	<5	7 ± 1^*
	0.250	7 ± 2^*	13 ± 2^†
	0.500	9 ± 2^*	25 ± 2^†
Palmitate	0.125	5 ± 1^*	15 ± 2^†
	0.250	12 ± 2^†	43 ± 3^†
	0.500	54 ± 5^†	94 ± 2^†
Oleate	0.125	<5	<5^‡^§
Palmitate	0.125
Oleate	0.250	7 ± 1^∥	13 ± 3^¶
Palmitate	0.250
Bromopalmitate	0.125	5 ± 1^#	37 ± 3^†
	0.250	13 ± 2^†	77 ± 1^†
	0.500	75 ± 6^†	100 ± 0^†

Data are means ± SE for six to nine experiments. FFA-induced toxicity after 2 and 8 days of culture at 10 mmol/l glucose and 1% bovine serum albumin. Cytotoxicity of single fatty acids was assessed by comparison with control condition (no fatty acids added):

P < 0.01;

†

P < 0.001;

‡

P < 0.01 vs. condition without palmitate;

P < 0.01;

∥

P < 0.05;

P < 0.001 vs. condition without oleate;

P < 0.05. (analysis of variance, followed by paired t test with Bonferroni correction).

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