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TABLE 3

FFA-induced apoptosis and necrosis in islet non–β-cells

Exposure time (mmol/l)Cytotoxicity
2 days
8 days
NecrosisApoptosisNecrosisApoptosis
Oleate 0.125 <5 5 ± 1* <5 24 ± 3* 
 0.250 <5 8 ± 2 <5 31 ± 5* 
 0.500 <5 10 ± 3 <5 27 ± 4* 
Palmitate 0.125 <5 <5 <5 6 ± 4 
 0.250 <5 <5 <5 17 ± 5 
 0.500 5 ± 3 12 ± 2*  5 ± 2 35 ± 7* 
Bromopalmitate 0.125 <5 10 ± 5 <5 51 ± 5 
 0.250 <5 13 ± 4 <5 56 ± 6 
 0.500 6 ± 3 22 ± 4* 14 ± 5 39 ± 8* 
Exposure time (mmol/l)Cytotoxicity
2 days
8 days
NecrosisApoptosisNecrosisApoptosis
Oleate 0.125 <5 5 ± 1* <5 24 ± 3* 
 0.250 <5 8 ± 2 <5 31 ± 5* 
 0.500 <5 10 ± 3 <5 27 ± 4* 
Palmitate 0.125 <5 <5 <5 6 ± 4 
 0.250 <5 <5 <5 17 ± 5 
 0.500 5 ± 3 12 ± 2*  5 ± 2 35 ± 7* 
Bromopalmitate 0.125 <5 10 ± 5 <5 51 ± 5 
 0.250 <5 13 ± 4 <5 56 ± 6 
 0.500 6 ± 3 22 ± 4* 14 ± 5 39 ± 8* 

Data are means ± SE for five experiments. Apoptosis and necrosis indexes after a 2- and 8-day FFA exposure of single non–β-cells in culture medium containing 6 mmol/l glucose, 1% bovine serum albumin, and 0.5 μg/ml growth hormone are shown. Statistical significance (FFA conditions vs. control) was calculated by analysis of variance A, followed by paired t test with the Bonferroni correction.

*

P < 0.01;

P < 0.05;

P < 0.001.

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