Effects of the src-inhibitor PP1 on VCAM-1 and ICAM-1 expression upon gly-LDL and dm-LDL stimulation
| . | VCAM-1 expression . | ICAM-1 expression . |
|---|---|---|
| Control | 35.79 ± 5.72 | 25.15 ± 1.30 |
| gly-LDL | 61.07 ± 1.51* | 26.33 ± 1.00 (NS) |
| gly-LDL + PP1 | 55.10 ± 0.30 (NS) | 25.80 ± 3.68 (NS) |
| dm-LDL | 59.21 ± 2.50* | 27.65 ± 3.22 (NS) |
| dm-LDL + PP1 | 56.11 ± 1.25 (NS) | 26.80 ± 2.30 (NS) |
| TNF-α | 59.20 ± 2.55* | 78.40 ± 5.33* |
| . | VCAM-1 expression . | ICAM-1 expression . |
|---|---|---|
| Control | 35.79 ± 5.72 | 25.15 ± 1.30 |
| gly-LDL | 61.07 ± 1.51* | 26.33 ± 1.00 (NS) |
| gly-LDL + PP1 | 55.10 ± 0.30 (NS) | 25.80 ± 3.68 (NS) |
| dm-LDL | 59.21 ± 2.50* | 27.65 ± 3.22 (NS) |
| dm-LDL + PP1 | 56.11 ± 1.25 (NS) | 26.80 ± 2.30 (NS) |
| TNF-α | 59.20 ± 2.55* | 78.40 ± 5.33* |
Data are means ± SEM of % fluorescent cells of 6 individual experiments. ECs were incubated with vehicle alone, with gly-LDL or with dm-LDL (100μg/ml), with or without PP1 (5umol/l). Tumor necrosis factor-α (TNF-α) (10nmol/l) was used as positive control. The cells were stained with an anti-VCAM antibody or with an anti-ICAM antibody. The expression of the corresponding protein was evaluated by fluorescence-activated cell sorter. ANOVA with Newman-Keuls multicomparison test was performed for control vs. experimental groups.
P < 0.05; NS not significant, P > 0.05.