American Diabetes Association

TABLE 2

Immunophenotype of LEW.1WR1 rats

Tissue	Phenotype (%)	Strain
Tissue	Phenotype (%)			LEW.1WR1	BBDR	LEW
Spleen	TCR⁺	36 ± 5	35 ± 4	45 ± 5^*
	TCR⁺ CD8⁺	12 ± 2	12 ± 1	16 ± 3^*
	TCR⁺ CD4⁺	23 ± 4	21 ± 4	26 ± 1
	TCR⁺ ART2.1⁺	27 ± 8	25 ± 9	30 ± 4
	CD25⁺ in the CD4⁺ population	8 ± 3	9 ± 2	11 ± 2
Cervical lymph nodes	TCR⁺	66 ± 5	72 ± 4	61 ± 3^†
	TCR⁺ CD8⁺	23 ± 3	21 ± 1	16 ± 2^*
	TCR⁺ CD4⁺	38 ± 5	48 ± 4^‡	36 ± 2
	TCR⁺ ART2.1⁺	40 ± 5	39 ± 6	36 ± 2
	CD25⁺ in the CD4⁺ population	9 ± 1	10 ± 1	9 ± 1
Mesenteric lymph nodes	TCR⁺	71 ± 14	76 ± 8	71 ± 2
	TCR⁺ CD8⁺	21 ± 6	22 ± 7	17 ± 1
	TCR⁺ CD4⁺	48 ± 10	52 ± 5	46 ± 3
	TCR⁺ ART2.1⁺	50 ± 12	47 ± 7	45 ± 3
	CD25⁺ in the CD4⁺ population	7 ± 1^§	9 ± 1^§	10 ± 1^§
Pancreatic lymph nodes	TCR⁺	71 ± 9	78 ± 1	75 ± 6
	TCR⁺ CD8⁺	20 ± 3	25 ± 3^‡	16 ± 1
	TCR⁺ CD4⁺	49 ± 10	50 ± 11	55 ± 6
	TCR⁺ ART2.1⁺	43 ± 8	42 ± 7	33 ± 10
	CD25⁺ in the CD4⁺ population	10 ± 4	13 ± 5	10 ± 1

Tissue	Phenotype (%)	Strain
Tissue	Phenotype (%)			LEW.1WR1	BBDR	LEW
Spleen	TCR⁺	36 ± 5	35 ± 4	45 ± 5^*
	TCR⁺ CD8⁺	12 ± 2	12 ± 1	16 ± 3^*
	TCR⁺ CD4⁺	23 ± 4	21 ± 4	26 ± 1
	TCR⁺ ART2.1⁺	27 ± 8	25 ± 9	30 ± 4
	CD25⁺ in the CD4⁺ population	8 ± 3	9 ± 2	11 ± 2
Cervical lymph nodes	TCR⁺	66 ± 5	72 ± 4	61 ± 3^†
	TCR⁺ CD8⁺	23 ± 3	21 ± 1	16 ± 2^*
	TCR⁺ CD4⁺	38 ± 5	48 ± 4^‡	36 ± 2
	TCR⁺ ART2.1⁺	40 ± 5	39 ± 6	36 ± 2
	CD25⁺ in the CD4⁺ population	9 ± 1	10 ± 1	9 ± 1
Mesenteric lymph nodes	TCR⁺	71 ± 14	76 ± 8	71 ± 2
	TCR⁺ CD8⁺	21 ± 6	22 ± 7	17 ± 1
	TCR⁺ CD4⁺	48 ± 10	52 ± 5	46 ± 3
	TCR⁺ ART2.1⁺	50 ± 12	47 ± 7	45 ± 3
	CD25⁺ in the CD4⁺ population	7 ± 1^§	9 ± 1^§	10 ± 1^§
Pancreatic lymph nodes	TCR⁺	71 ± 9	78 ± 1	75 ± 6
	TCR⁺ CD8⁺	20 ± 3	25 ± 3^‡	16 ± 1
	TCR⁺ CD4⁺	49 ± 10	50 ± 11	55 ± 6
	TCR⁺ ART2.1⁺	43 ± 8	42 ± 7	33 ± 10
	CD25⁺ in the CD4⁺ population	10 ± 4	13 ± 5	10 ± 1

Data are means ± SD of positively stained cells from five to six individual rats. Nondiabetic, unmanipulated LEW.1WR1, BBDR, and LEW rats 35–42 days of age were processed for immunophenotyping as described in research design and methods. The LEW.1WR1 and BBDR rats were of both sexes; the LEW rats were all male. Statistical analysis by one-way ANOVA revealed a number of statistical differences for various phenotypes scattered through the dataset, but there were no consistent differences that appeared to indicate biological differences among the strains. The ART2 surface alloantigen in the rat was formerly designated RT6.

P < 0.05 vs. BBDR and LEW.1WR1;

†

P < 0.05 vs. BBDR;

‡

P < 0.05 vs. LEW and LEW.1WR1;

P < 0.05 vs. both other strains. No other pairwise comparisons among the three strains were statistically significant.

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