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TABLE 1

Quantitative analysis of relative GFP fluorescence in islet cells

AAV1AAV2AAV5AAV6AAV8
In vitro 85 ± 8.9 19.7 ± 4.7 3.5 ± 0.4 100 ± 15.6 20.1 ± 3.7 
Intraperitoneal 17.4 ± 2.9 1.5 ± 0.9 2.0 ± 0.7 36.7 ± 15.1 100 ± 12.2 
Intraductal ND 6.4 ± 2.9 ND 100 ± 23.9 65.8 ± 4.3 
Intravenous + liver blockade ND ND ND 35.5 ± 17.5 100 ± 3.3 
AAV1AAV2AAV5AAV6AAV8
In vitro 85 ± 8.9 19.7 ± 4.7 3.5 ± 0.4 100 ± 15.6 20.1 ± 3.7 
Intraperitoneal 17.4 ± 2.9 1.5 ± 0.9 2.0 ± 0.7 36.7 ± 15.1 100 ± 12.2 
Intraductal ND 6.4 ± 2.9 ND 100 ± 23.9 65.8 ± 4.3 
Intravenous + liver blockade ND ND ND 35.5 ± 17.5 100 ± 3.3 

Data are means ± SE. Each data point contained three to four mice. Quantitative analysis of GFP fluorescence intensities was obtained using the Metamorph Software. For each delivery route, values were expressed as relative GFP fluorescence intensity achieved by different serotype AAV vectors, thus, as percentage of the highest fluorescence level (labeled in bold) achieved by a specific serotype. The experimental protocols and corresponding photographs can be found elsewhere (in vitro infection and intraperitoneal administration seen in Fig. 1, intraductal seen in Fig. 3B, and intravenous + liver blockade seen in Fig. 5). ND, not determined.

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