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TABLE 4

Expression of lipid metabolism genes in WAT, liver, and skeletal muscle in AR−/y mice as quantified by real-time RT-PCR

GeneΔΔCT (average ΔCT − average ΔCT.WT)Normalized amount relative to wild type 2−ΔΔCT
WAT   
    PPARγ −1.40 ± 0.81 2.6 (1.5–4.6) 
    C/EBPα −3.98 ± 0.22 15.8 (13.5–18.4) 
    SREBP1c −2.28 ± 0.58 4.9 (3.2–7.3) 
    aP2 −0.43 ± 0.17 1.35 (1.20–1.52) 
Liver   
    PPARα 3.13 ± 0.45 0.11 (0.08–0.16) 
Skeletal muscle   
    PPARα 2.36 ± 0.13 0.19 (0.18–0.21) 
GeneΔΔCT (average ΔCT − average ΔCT.WT)Normalized amount relative to wild type 2−ΔΔCT
WAT   
    PPARγ −1.40 ± 0.81 2.6 (1.5–4.6) 
    C/EBPα −3.98 ± 0.22 15.8 (13.5–18.4) 
    SREBP1c −2.28 ± 0.58 4.9 (3.2–7.3) 
    aP2 −0.43 ± 0.17 1.35 (1.20–1.52) 
Liver   
    PPARα 3.13 ± 0.45 0.11 (0.08–0.16) 
Skeletal muscle   
    PPARα 2.36 ± 0.13 0.19 (0.18–0.21) 

CT, threshold cycle; CT.WT, threshold cycle for wild type.

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