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TABLE 1

mRNA content of select genes in human myotubes determined using RT-PCR

GeneScrambledsiMCD
FATP-1 0.98 ± 0.25 1.22 ± 0.29* 
PDK-4 0.99 ± 0.16 0.89 ± 0.24 
AMPK-α1 1.02 ± 0.10 1.00 ± 0.09 
AMPK-α2 0.96 ± 0.24 0.74 ± 0.13 
ACC-α 0.98 ± 0.24 0.87 ± 0.18 
ACC-β 0.98 ± 0.32 1.12 ± 0.27 
GeneScrambledsiMCD
FATP-1 0.98 ± 0.25 1.22 ± 0.29* 
PDK-4 0.99 ± 0.16 0.89 ± 0.24 
AMPK-α1 1.02 ± 0.10 1.00 ± 0.09 
AMPK-α2 0.96 ± 0.24 0.74 ± 0.13 
ACC-α 0.98 ± 0.24 0.87 ± 0.18 
ACC-β 0.98 ± 0.32 1.12 ± 0.27 

Data are means ± SE. Cells were transfected with siRNA against a scrambled sequence or MCD (siMCD). Results are reported as percentage of control (nontransfected myotubes) and normalized to glyceraldehyde-3-phosphate dehydrogenase. Paired Student's t test comparisons were made between groups (scrambled vs. siMCD).

*

Significantly different from scrambled (P < 0.05).

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