Fat pad mass, tissue triglyceride levels, and metabolic parameters from hyperinsulinemic-euglycemic clamps in rats
| . | Control . | HFD . | HFD dhBBR . |
|---|---|---|---|
| Body mass (g) | 349 ± 7 | 367 ± 5* | 361 ± 6 |
| Epididymal fat pad (%) | 1.05 ± 0.04 | 1.94 ± 0.11† | 1.48 ± 0.10†§ |
| Inguinal fat pad (%) | 1.04 ± 0.07 | 2.28 ± 0.16† | 1.79 ± 0.11†§ |
| Muscle triglyceride (μmol/g) | 3.0 ± 0.3 | 5.1 ± 1.0* | 3.1 ± 0.2‡ |
| Liver triglyceride (μmol/g) | 4.8 ± 0.2 | 17.1 ± 1.9† | 9.4 ± 1.4* |
| Glucose infusion rate (mg · kg−1 · min−1) | 40.2 ± 2.2 | 19.0 ± 0.4† | 27.4 ± 1.9†§ |
| Rd (mg · kg−1 · min−1) | 38.4 ± 1.9 | 23.2 ± 0.5† | 30.3 ± 0.6†§ |
| Hepatic glucose output (mg · kg−1 · min−1) | −1.7 ± 0.5 | 4.2 ± 0.6† | 2.8 ± 1.7† |
| Rg′: red gastrocnemius | 35.6 ± 3.3 | 19.8 ± 1.7† | 34.9 ± 7.9‡ |
| Rg′: white gastrocnemius | 15.2 ± 2.7 | 5.7 ± 0.7† | 8.5 ± 1.5* |
| Rg′: epididymal fat | 1.8 ± 0.2 | 1.3 ± 0.1 | 2.1 ± 0.4‡ |
| . | Control . | HFD . | HFD dhBBR . |
|---|---|---|---|
| Body mass (g) | 349 ± 7 | 367 ± 5* | 361 ± 6 |
| Epididymal fat pad (%) | 1.05 ± 0.04 | 1.94 ± 0.11† | 1.48 ± 0.10†§ |
| Inguinal fat pad (%) | 1.04 ± 0.07 | 2.28 ± 0.16† | 1.79 ± 0.11†§ |
| Muscle triglyceride (μmol/g) | 3.0 ± 0.3 | 5.1 ± 1.0* | 3.1 ± 0.2‡ |
| Liver triglyceride (μmol/g) | 4.8 ± 0.2 | 17.1 ± 1.9† | 9.4 ± 1.4* |
| Glucose infusion rate (mg · kg−1 · min−1) | 40.2 ± 2.2 | 19.0 ± 0.4† | 27.4 ± 1.9†§ |
| Rd (mg · kg−1 · min−1) | 38.4 ± 1.9 | 23.2 ± 0.5† | 30.3 ± 0.6†§ |
| Hepatic glucose output (mg · kg−1 · min−1) | −1.7 ± 0.5 | 4.2 ± 0.6† | 2.8 ± 1.7† |
| Rg′: red gastrocnemius | 35.6 ± 3.3 | 19.8 ± 1.7† | 34.9 ± 7.9‡ |
| Rg′: white gastrocnemius | 15.2 ± 2.7 | 5.7 ± 0.7† | 8.5 ± 1.5* |
| Rg′: epididymal fat | 1.8 ± 0.2 | 1.3 ± 0.1 | 2.1 ± 0.4‡ |
Data are means ± SE (n = 5–12 per group). dhBBR was provided in the HFD (100 mg · kg−1 · day−1) for the final 2 weeks of high-fat feeding. Fat pad weights are expressed as a percentage of body mass. Plasma levels of glucose and insulin were similar among the three groups during the clamp (data not shown). Rg′, insulin-stimulated glucose uptake during the clamp (μmol/100 g · min).
P < 0.05,
P < 0.01 vs. control;
P < 0.05,
P < 0.01 vs. HFD.