American Diabetes Association

TABLE 5

ATGL, HSL, LPL mRNA expression relative to GADPH, and GLUT-4 mRNA expression relative to β-actin in SCAAT

		CON		FDR
		Before BR rest (basal n = 16; insulin stimulation n = 14)	After BR (basal n = 16; insulin stimulation n = 16)	Before BR (basal n = 10; insulin stimulation n = 11)	After BR rest (basal n = 10; insulin stimulation n = 11)
ATGL**	Basal	0.10 ± 0.02*	0.10 ± 0.01	0.04 ± 0.02*‡	0.06 ± 0.04
ATGL**	Insulin stimulation	0.12 ± 0.03	0.11 ± 0.02	0.08 ± 0.02	0.07 ± 0.01
HSL**	Basal	0.10 ± 0.02*	0.15 ± 0.04*	0.02 ± 0.01*‡	0.01 ± 0.01*‡
HSL**	Insulin stimulation	0.08 ± 0.01	0.09 ± 0.02	0.09 ± 0.03	0.06 ± 0.02
LPL**	Basal	1.2 ± 0.2*†	2.3 ± 0.4*†	0.4 ± 0.1*‡	0.3 ± 0.2*‡
LPL**	Insulin stimulation	1.8 ± 0.4	1.7 ± 0.2	1.0 ± 0.2	1.1 ± 0.3
GLUT 4**	Basal	0.9 ± 0.3	0.6 ± 0.1‡	1.0 ± 0.3	1.0 ± 0.3‡
GLUT 4**	Insulin stimulation	0.8 ± 0.2	1.2 ± 0.2	0.6 ± 0.2	2.3 ± 0.8

		CON		FDR
		Before BR rest (basal n = 16; insulin stimulation n = 14)	After BR (basal n = 16; insulin stimulation n = 16)	Before BR (basal n = 10; insulin stimulation n = 11)	After BR rest (basal n = 10; insulin stimulation n = 11)
ATGL**	Basal	0.10 ± 0.02*	0.10 ± 0.01	0.04 ± 0.02*‡	0.06 ± 0.04
ATGL**	Insulin stimulation	0.12 ± 0.03	0.11 ± 0.02	0.08 ± 0.02	0.07 ± 0.01
HSL**	Basal	0.10 ± 0.02*	0.15 ± 0.04*	0.02 ± 0.01*‡	0.01 ± 0.01*‡
HSL**	Insulin stimulation	0.08 ± 0.01	0.09 ± 0.02	0.09 ± 0.03	0.06 ± 0.02
LPL**	Basal	1.2 ± 0.2*†	2.3 ± 0.4*†	0.4 ± 0.1*‡	0.3 ± 0.2*‡
LPL**	Insulin stimulation	1.8 ± 0.4	1.7 ± 0.2	1.0 ± 0.2	1.1 ± 0.3
GLUT 4**	Basal	0.9 ± 0.3	0.6 ± 0.1‡	1.0 ± 0.3	1.0 ± 0.3‡
GLUT 4**	Insulin stimulation	0.8 ± 0.2	1.2 ± 0.2	0.6 ± 0.2	2.3 ± 0.8

Data are mean ± SE.

*Significant difference CON vs. FDR, P < 0.05.

†Significant difference before vs. after BR within the group; P < 0.05.

‡Significant difference basal vs. insulin-stimulated state within the group on the specified day, P < 0.05.

**Data were log transformed before statistical test.