Clinical, metabolic, and skeletal muscle characteristics
| . | Lean . | Obese . |
|---|---|---|
| N | 20 | 14 |
| Male/female | 11/9 | 7/7 |
| Age (years) | 38 ± 3 | 38 ± 4 |
| BMI (kg/m2) | 25 ± 1 | 36 ± 1* |
| Weight (kg) | 77.6 ± 3.6 | 103.1 ± 4.2 |
| Fat mass (kg) | 20.4 ± 2.8 | 37.4 ± 2.5* |
| Plasma glucose (mg/dl) | 86 ± 2 | 93 ± 2† |
| Serum insulin (pmol/l) | 30 ± 6 | 90 ± 12* |
| A1C (%) | 5.4 ± 0 | 5.7 ± 0.1* |
| HDL cholesterol (mmol/l) | 51 ± 3 | 46 ± 2 |
| LDL cholesterol (mmol/l) | 109 ± 9 | 113 ± 8 |
| Plasma triglycerides (mmol/l) | 85 ± 8 | 126 ± 21† |
| OGTT (2-h glycemia) | 105 ± 6 | 135 ± 8* |
| Rd basal (mg · kg−1 · min−1) | 2.1 ± 0.1 | 1.8 ± 0.1* |
| Rd clamp (mg · kg−1 · min−1) | 7.9 ± 0.6 | 3.7 ± 0.3* |
| HGP clamp (mg · kg−1 · min−1) | 0.1 ± 0.1 | 0.0 ± 0.1 |
| Pulse (bpm) | 67 ± 2 | 71 ± 2 |
| Systolic/diastolic blood pressure (mmHg) | 124 ± 5/82 ± 3 | 130 ± 3/87 ± 2 |
| Vo2max (ml · min−1 · kg−1) | 31.5 ± 1.9 | 25.9 ± 1.8 |
| Vo2max (ml · min−1 · kg FFM−1) | 42.9 ± 2.4 | 38.8 ± 2.0 |
| % MHC I | 31 ± 4 | 24 ± 3 |
| % MHC IIx | 21 ± 2 | 31 ± 4* |
| % MHC IIa | 48 ± 6 | 45 ± 6 |
| Isolated mitochondrial protein (μg/μl)‡ | 6.5 ± 0.3 | 5.4 ± 0.2* |
| Isolated citrate synthase activity (μmol · min−1 · g wet weight−1) | 8.2 ± 0.6 | 6.0 ± 0.2* |
| Mitochondria recovery (%) | 66 ± 3 | 64 ± 3 |
| . | Lean . | Obese . |
|---|---|---|
| N | 20 | 14 |
| Male/female | 11/9 | 7/7 |
| Age (years) | 38 ± 3 | 38 ± 4 |
| BMI (kg/m2) | 25 ± 1 | 36 ± 1* |
| Weight (kg) | 77.6 ± 3.6 | 103.1 ± 4.2 |
| Fat mass (kg) | 20.4 ± 2.8 | 37.4 ± 2.5* |
| Plasma glucose (mg/dl) | 86 ± 2 | 93 ± 2† |
| Serum insulin (pmol/l) | 30 ± 6 | 90 ± 12* |
| A1C (%) | 5.4 ± 0 | 5.7 ± 0.1* |
| HDL cholesterol (mmol/l) | 51 ± 3 | 46 ± 2 |
| LDL cholesterol (mmol/l) | 109 ± 9 | 113 ± 8 |
| Plasma triglycerides (mmol/l) | 85 ± 8 | 126 ± 21† |
| OGTT (2-h glycemia) | 105 ± 6 | 135 ± 8* |
| Rd basal (mg · kg−1 · min−1) | 2.1 ± 0.1 | 1.8 ± 0.1* |
| Rd clamp (mg · kg−1 · min−1) | 7.9 ± 0.6 | 3.7 ± 0.3* |
| HGP clamp (mg · kg−1 · min−1) | 0.1 ± 0.1 | 0.0 ± 0.1 |
| Pulse (bpm) | 67 ± 2 | 71 ± 2 |
| Systolic/diastolic blood pressure (mmHg) | 124 ± 5/82 ± 3 | 130 ± 3/87 ± 2 |
| Vo2max (ml · min−1 · kg−1) | 31.5 ± 1.9 | 25.9 ± 1.8 |
| Vo2max (ml · min−1 · kg FFM−1) | 42.9 ± 2.4 | 38.8 ± 2.0 |
| % MHC I | 31 ± 4 | 24 ± 3 |
| % MHC IIx | 21 ± 2 | 31 ± 4* |
| % MHC IIa | 48 ± 6 | 45 ± 6 |
| Isolated mitochondrial protein (μg/μl)‡ | 6.5 ± 0.3 | 5.4 ± 0.2* |
| Isolated citrate synthase activity (μmol · min−1 · g wet weight−1) | 8.2 ± 0.6 | 6.0 ± 0.2* |
| Mitochondria recovery (%) | 66 ± 3 | 64 ± 3 |
Data are presented as means ± SE. Student's t test was performed.
*P < 0.01 and
†P < 0.05 vs. lean participants.
‡Mitochondria pellets were resuspended in 100 μl of mannitol-sucrose buffer. HGP, hepatic glucose production.