American Diabetes Association

TABLE 2

Binding of preproinsulin peptides to selected HLA class I alleles

Peptide^*	Sequence	HLA I	HLA motif^†	Anchoring fit^‡	Binding^§			Type of binder^¶
Peptide^*	Sequence	HLA I	HLA motif^†	Anchoring fit^‡				Type of binder^¶	10⁻⁴	10⁻⁶
1–8	MALWMRLL	A2	MALWMRLL	+	46	0	L
2–11	ALWMRLLPLL	A2	ALWMRLLPLL	++	55	53	H
		A24	ALWMRLLPLL	+	80	100	H
		B8	ALWMRLLPLL	+	24	20	I
6–14	RLLPLLALL	A2	RLLPLLALL	++	36	65	H
6–16	RLLPLLALLAL	A2	RLLPLLALLAL	++	96	71	H
14–23	LALWGPDPAA	A2	LALWGPDPAA	+	44	36	I
15–24	ALWGPDPAAA	A2	ALWGPDPAAA	+	96	94	H
15–25	ALWGPDPAAAF	A1	ALWGPDPAAAF	+	18	41	I
		A24	ALWGPDPAAAF	++	82	100	H

Peptide^*	Sequence	HLA I	HLA motif^†	Anchoring fit^‡	Binding^§			Type of binder^¶
Peptide^*	Sequence	HLA I	HLA motif^†	Anchoring fit^‡				Type of binder^¶	10⁻⁴	10⁻⁶
1–8	MALWMRLL	A2	MALWMRLL	+	46	0	L
2–11	ALWMRLLPLL	A2	ALWMRLLPLL	++	55	53	H
		A24	ALWMRLLPLL	+	80	100	H
		B8	ALWMRLLPLL	+	24	20	I
6–14	RLLPLLALL	A2	RLLPLLALL	++	36	65	H
6–16	RLLPLLALLAL	A2	RLLPLLALLAL	++	96	71	H
14–23	LALWGPDPAA	A2	LALWGPDPAA	+	44	36	I
15–24	ALWGPDPAAA	A2	ALWGPDPAAA	+	96	94	H
15–25	ALWGPDPAAAF	A1	ALWGPDPAAAF	+	18	41	I
		A24	ALWGPDPAAAF	++	82	100	H

Data are percent.

NH₂- and COOH-terminal positions in preproinsulin.

†

Anchoring positions are in bold characters.

‡

HLA anchoring motifs: ++, strict; +, tolerated.

Binding (%) = binding of tested peptide/binding of viral reference peptide.

L, low binder (binding <20% at 10⁻⁶ mol/l and binding >20% at 10⁻⁴ mol/l); I, intermediate binder (binding between 20 and 50% at 10⁻⁶ mol/l); H, high binder (binding >50% at 10⁻⁶ mol/l).

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