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TABLE 2

Tryptophan measurements of media from adenovirus-infected islets

NegAd-LacZAd-IDO
RPMI (control) 16.3 ± 0.8 — — 
Day 8 13.3 ± 0.5 14.4 ± 0.8 6.7 ± 1.0 
Day 14 15.2 ± 0.8 10.4 ± 0.3 0 (−1.4) ± 0.4 
NegAd-LacZAd-IDO
RPMI (control) 16.3 ± 0.8 — — 
Day 8 13.3 ± 0.5 14.4 ± 0.8 6.7 ± 1.0 
Day 14 15.2 ± 0.8 10.4 ± 0.3 0 (−1.4) ± 0.4 

Data are means ± SD. In this representative experiment, groups of 400 islets were infected with Ad-LacZ or Ad-IDO or used uninfected (Neg) and cultured for 2 weeks. Samples of media were taken at 8 and 14 days for analysis of tryptophan content. Tryptophan is expressed in nanomoles per milliliter.

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