Glucose-stimulated insulin secretion of infected islets in vitro
| Treatment . | |||
|---|---|---|---|
| Virus . | 3 mmol/l glucose . | 20 mmol/l glucose . | 3 mmol/l glucose . |
| Neg | 295 ± 26.2 | 1241 ± 26.2 | 327 ± 23.3 |
| Ad-LacZ | 237 ± 21.9 | 1201 ± 27.6 | 260 ± 39.6 |
| Ad-IDO | 288 ± 14.8 | 1174 ± 42.4 | 271 ± 26.2 |
| Treatment . | |||
|---|---|---|---|
| Virus . | 3 mmol/l glucose . | 20 mmol/l glucose . | 3 mmol/l glucose . |
| Neg | 295 ± 26.2 | 1241 ± 26.2 | 327 ± 23.3 |
| Ad-LacZ | 237 ± 21.9 | 1201 ± 27.6 | 260 ± 39.6 |
| Ad-IDO | 288 ± 14.8 | 1174 ± 42.4 | 271 ± 26.2 |
Data are means ± SD. In this experiment, shown as an example, groups of 25 islets were infected with Ad-LacZ and Ad-IDO. Infected islets were compared with uninfected (Neg) islets by culturing them for 4 days before being pulsed with media containing high and low glucose levels. Media were collected after a 30-min exposure to islets. Insulin content measured by ELISA is expressed as picogram insulin per milliliter of media.