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TABLE 4

Tryptophan levels in a “head start” proliferation assay

100 μl media
200 μl media
NegAd-LacZAd-IDONegAd-LacZAd-IDO
Day 5 14.8 ± 0.3 14.2 ± 1.9 8.7 ± 0.4 19.0 ± 3.1 19.2 ± 0.6 21.6 ± 0.6 
Day 6 14.3 ± 1.1 15.7 ± 2.1 10.3 ± 5.6 19.9 ± 2.1 20.7 ± 3.1 19.0 ± 2.7 
Day 7 16.2 ± 1.3 16.9 ± 1.1 8.3 ± 2.0 24.7 ± 2.2 22.3 ± 2.1 16.9 ± 5.1 
Day 8 16.4 ± 0.2 14.4 ± 0.3 7.0 ± 0.8 16.7 ± 0.6 19.0 ± 0.1 15.8 ± 1.4 
100 μl media
200 μl media
NegAd-LacZAd-IDONegAd-LacZAd-IDO
Day 5 14.8 ± 0.3 14.2 ± 1.9 8.7 ± 0.4 19.0 ± 3.1 19.2 ± 0.6 21.6 ± 0.6 
Day 6 14.3 ± 1.1 15.7 ± 2.1 10.3 ± 5.6 19.9 ± 2.1 20.7 ± 3.1 19.0 ± 2.7 
Day 7 16.2 ± 1.3 16.9 ± 1.1 8.3 ± 2.0 24.7 ± 2.2 22.3 ± 2.1 16.9 ± 5.1 
Day 8 16.4 ± 0.2 14.4 ± 0.3 7.0 ± 0.8 16.7 ± 0.6 19.0 ± 0.1 15.8 ± 1.4 

Data are means ± SD. Islets not cultured with spleen cells were plated in parallel to islets co-cultured with spleen cells to test tryptophan levels of culture media (nmole/ml) on days corresponding to T-cell proliferation measurements in media volumes of 100 and 200 μl per well. Uninfected islets (Neg) were compared with islets infected with Ad-LacZ or Ad-IDO.

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