American Diabetes Association

TABLE 3

Primer sequences and PCR conditions for mutation analysis of SLC2A10

Primer	Sequence, 5′→3′	Location	T_anneal (°C)	MgCl₂ (mmol/l)
G10F1.1^*	AGG GCA GGA GGG ACA GAG	exon 1	55	1
G10R1.1^*	CAC CCG AGA GGC GGT ATC
G10F2.1	GGA TGG TAT GAC AAG GAA CCA	exon 2	55	2
G10R2.1	CAA GTT GCT CCC GAG GAT G
G10F2.2	GGT GGC TTC CTC ATT GAC TG	exon 2	55	3
G10R2.2	ATC CCC AGG GGG TAC CAG
G10F2.3	TGC TCT CCT ATG CCC TCA AC	exon 2	55	2
G10R2.3	GCT GCC CTG TTA GTT GCT G
G10F2.4	CAG GGC ACG CGA TAA CAT	exon 2	55	2
G10R2.4	CAC GGC AAA GCT GAC GAG
G10F2.5	TCT GTT GCT AGC TGG CTG TG	exon 2	55	2
G10R2.5	GGC TGA GGG GTC TCC AGA T
G10F2.6	AGC CAA TCT TGT CCA CTG CT	exon 2	55	2
G10R2.6	ACC CTG CCA GGT CAT CAG
G10F3.1	GAA AAT CCC ATT GTT GAG AGG	exon 3	55	1
G10R3.1	TGC TTT AGA GTA GGG AGC TTG G
G10F4.1	TCC ATG CCT GAC CTA GAA CC	exon 4	55	2
G10R4.1	AAA ATC CTG AAG CTG TGT GC
G10F5.1	GAA CCC CAG TGG AAG GTC	exon 5	55	2
G10R5.1	CAG CAA AAG CAG ACC ACC T

Primer	Sequence, 5′→3′	Location	T_anneal (°C)	MgCl₂ (mmol/l)
G10F1.1^*	AGG GCA GGA GGG ACA GAG	exon 1	55	1
G10R1.1^*	CAC CCG AGA GGC GGT ATC
G10F2.1	GGA TGG TAT GAC AAG GAA CCA	exon 2	55	2
G10R2.1	CAA GTT GCT CCC GAG GAT G
G10F2.2	GGT GGC TTC CTC ATT GAC TG	exon 2	55	3
G10R2.2	ATC CCC AGG GGG TAC CAG
G10F2.3	TGC TCT CCT ATG CCC TCA AC	exon 2	55	2
G10R2.3	GCT GCC CTG TTA GTT GCT G
G10F2.4	CAG GGC ACG CGA TAA CAT	exon 2	55	2
G10R2.4	CAC GGC AAA GCT GAC GAG
G10F2.5	TCT GTT GCT AGC TGG CTG TG	exon 2	55	2
G10R2.5	GGC TGA GGG GTC TCC AGA T
G10F2.6	AGC CAA TCT TGT CCA CTG CT	exon 2	55	2
G10R2.6	ACC CTG CCA GGT CAT CAG
G10F3.1	GAA AAT CCC ATT GTT GAG AGG	exon 3	55	1
G10R3.1	TGC TTT AGA GTA GGG AGC TTG G
G10F4.1	TCC ATG CCT GAC CTA GAA CC	exon 4	55	2
G10R4.1	AAA ATC CTG AAG CTG TGT GC
G10F5.1	GAA CCC CAG TGG AAG GTC	exon 5	55	2
G10R5.1	CAG CAA AAG CAG ACC ACC T

When amplifying exon 1 (using primers G10F1.1 and G10R1.1), 7.5% DMSO was included in the reaction mix.