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TABLE 1

Ad-MafB activates endogenous glucagon gene transcription in βTC-3 cells and not NIH3T3

Ad-GFPAd-MafB
10×50×
βTC-3     
    MafB 1.00 45.52 1,230.57 18,260.62 
    Glucagon 1.00 2.02 3.48 7.83 
    Insulin I 1.00 1.29 1.37 1.30 
    Insulin II 1.00 1.09 1.04 1.00 
    pdx1 1.00 1.49 0.83 0.81 
    γ-Actin 1.00 1.17 0.78 0.88 
NIH3T3     
    MafB 1.00 27.61 143.99 1,486.43 
    Glucagon ND ND ND ND 
    Insulin I ND ND ND ND 
    pdx1 ND ND ND ND 
    γ-Actin 1.00 0.98 0.85 0.96 
Ad-GFPAd-MafB
10×50×
βTC-3     
    MafB 1.00 45.52 1,230.57 18,260.62 
    Glucagon 1.00 2.02 3.48 7.83 
    Insulin I 1.00 1.29 1.37 1.30 
    Insulin II 1.00 1.09 1.04 1.00 
    pdx1 1.00 1.49 0.83 0.81 
    γ-Actin 1.00 1.17 0.78 0.88 
NIH3T3     
    MafB 1.00 27.61 143.99 1,486.43 
    Glucagon ND ND ND ND 
    Insulin I ND ND ND ND 
    pdx1 ND ND ND ND 
    γ-Actin 1.00 0.98 0.85 0.96 

Data are expressed as the normalized fold difference relative to GFP alone (defined as 1). MafB and glucagon mRNA levels in βTC-3 and NIH 3T3 cells infected with Ad-GFP (MOI 100) or Ad-MafB (1× = MOI 2; 10× = MOI 20; 50× = MOI 100). All of the expression data were derived by real-time PCR analysis. A representative experiment is shown (n = 3). ND, not detected.

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