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TABLE 1

List of sequences of primers used

Product size (bp)Taq polymeraseAnnealing temperature (°C)
Promoter-1 forward 5′-TCAGAAGTCATCTTGCTACCAGTA-3′ 866 LA Taq 60 
Promoter-1 reverse 5′-ACTGAGTGCCACCCATGAGAACTG-3′    
Promoter-2 forward 5′-AGGAATTGGTTTCACGCTGAGGCA-3′ 1177 LA Taq 60 
Promoter-2 reverse 5′-CGCGCCGAGAAGGCATACACCGAC-3′    
    Promoter-2F-1 5′-ACTTCCTGGCCTAGCTCACCCTGG-3′    
    Promoter-2F-2 5′-GACCAGACATGCCTGCTGCTCCTT-3′    
Exon1-1 forward 5′-CGCTCCGCTGGCTTCTTGGACAATTG-3′ 384 AmpliTaq Gold 60 
Exon1-1 reverse 5′-GACTCTCCGCGCATGGAGCCGACG-3′    
Intorn1-1 forward 5′-GGTACTACTTATCGAGAATGTGGC-3′ 510 LA Taq 65 
Intorn1-1 reverse 5′-GCACTTCTCGAAGAGCATGAAGTT-3′    
Exon2-1 forward 5′-CTGTTTGTGGTTAGAACGTTTCTC-3′ 320 AmpliTaq Gold 60 
Exon2-1 reverse 5′-ACCAGGAACTTCTCAAAGTTCCAG-3′    
Exon2-2 forward 5′-CGCCACCGCGCTTATGACCG-3′ 187 AmpliTaq Gold 60 
Exon2-2 reverse 5′-GCAGCACTGCAACTGCCAAGCAG-3′    
Exon2-3 forward 5′-AAGTTCCTGGTGGGCCCTGACGGT-3′ 514 LA Taq 60 
Exon2-3 reverse 5′-CAGCTACTCAGGAGGTTGAGGCAG-3′    
Product size (bp)Taq polymeraseAnnealing temperature (°C)
Promoter-1 forward 5′-TCAGAAGTCATCTTGCTACCAGTA-3′ 866 LA Taq 60 
Promoter-1 reverse 5′-ACTGAGTGCCACCCATGAGAACTG-3′    
Promoter-2 forward 5′-AGGAATTGGTTTCACGCTGAGGCA-3′ 1177 LA Taq 60 
Promoter-2 reverse 5′-CGCGCCGAGAAGGCATACACCGAC-3′    
    Promoter-2F-1 5′-ACTTCCTGGCCTAGCTCACCCTGG-3′    
    Promoter-2F-2 5′-GACCAGACATGCCTGCTGCTCCTT-3′    
Exon1-1 forward 5′-CGCTCCGCTGGCTTCTTGGACAATTG-3′ 384 AmpliTaq Gold 60 
Exon1-1 reverse 5′-GACTCTCCGCGCATGGAGCCGACG-3′    
Intorn1-1 forward 5′-GGTACTACTTATCGAGAATGTGGC-3′ 510 LA Taq 65 
Intorn1-1 reverse 5′-GCACTTCTCGAAGAGCATGAAGTT-3′    
Exon2-1 forward 5′-CTGTTTGTGGTTAGAACGTTTCTC-3′ 320 AmpliTaq Gold 60 
Exon2-1 reverse 5′-ACCAGGAACTTCTCAAAGTTCCAG-3′    
Exon2-2 forward 5′-CGCCACCGCGCTTATGACCG-3′ 187 AmpliTaq Gold 60 
Exon2-2 reverse 5′-GCAGCACTGCAACTGCCAAGCAG-3′    
Exon2-3 forward 5′-AAGTTCCTGGTGGGCCCTGACGGT-3′ 514 LA Taq 60 
Exon2-3 reverse 5′-CAGCTACTCAGGAGGTTGAGGCAG-3′    

Two primers, promoter-2F-1 and -2F-2, were used only for sequencing.

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