Nuclear receptor and lipid metabolism enzyme activity, protein, and mRNA expression in wild-type and Akita mice
| . | Wild type . | Akita . | P value . |
|---|---|---|---|
| Fatty acid synthesis | |||
| SREBP-1 protein | 3.0 ± 0.2 | 5.4 ± 0.2 | <0.01 |
| SCD-1 mRNA | 1.8 ± 0.2 | 3.6 ± 0.3 | <0.05 |
| SCD-1 protein | 7.2 ± 0.1 | 11.6 ± 0.3 | <0.05 |
| SCD-1 activity | 2.5 ± 0.1 | 4.2 ± 0.3 | <0.05 |
| FXR mRNA | 9.1 ± 0.2 | 2.0 ± 0.4 | <0.01 |
| SHP mRNA | 3.9 ± 0.2 | 2.1 ± 0.4 | <0.05 |
| Cholesterol synthesis and efflux | |||
| SREBP-2 protein | 1.6 ± 0.2 | 3.4 ± 0.3 | <0.01 |
| ABCA1 mRNA | 14.8 ± 1.0 | 5.8 ± 0.9 | <0.01 |
| LXR-α | 12.3 ± 0.8 | 3.1 ± 0.7 | <0.01 |
| LXR-β | 12.9 ± 0.9 | 2.1 ± 0.8 | <0.01 |
| . | Wild type . | Akita . | P value . |
|---|---|---|---|
| Fatty acid synthesis | |||
| SREBP-1 protein | 3.0 ± 0.2 | 5.4 ± 0.2 | <0.01 |
| SCD-1 mRNA | 1.8 ± 0.2 | 3.6 ± 0.3 | <0.05 |
| SCD-1 protein | 7.2 ± 0.1 | 11.6 ± 0.3 | <0.05 |
| SCD-1 activity | 2.5 ± 0.1 | 4.2 ± 0.3 | <0.05 |
| FXR mRNA | 9.1 ± 0.2 | 2.0 ± 0.4 | <0.01 |
| SHP mRNA | 3.9 ± 0.2 | 2.1 ± 0.4 | <0.05 |
| Cholesterol synthesis and efflux | |||
| SREBP-2 protein | 1.6 ± 0.2 | 3.4 ± 0.3 | <0.01 |
| ABCA1 mRNA | 14.8 ± 1.0 | 5.8 ± 0.9 | <0.01 |
| LXR-α | 12.3 ± 0.8 | 3.1 ± 0.7 | <0.01 |
| LXR-β | 12.9 ± 0.9 | 2.1 ± 0.8 | <0.01 |
Data are means ± SE (n = 6 mice in each group).