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TABLE 1

Cytokine/chemokine secretion is associated with the Raf/MEK/ERK and NF-κB pathways in human and NHP islet cells

ControlCD40LPD98059 + CD40LBay 117082 + CD40L
NHP/1 (pg/ml)     
    IL-6 4.12 ± 2.61 10.18 ± 1.09* 5.10 ± 0.32 2.69 ± 0.37 
    IL-8 635 ± 248 1,701 ± 396* 463 ± 7.69 55 ± 1.88 
    MCP-1 181 ± 43 361 ± 57* 159 ± 8.30 17.39 ± 0.10 
    MIP-1β 2.55 ± 0.55 31.60 ± 2.59* 10.62 ± 0.24 1.20 ± 0.09 
Human islets (pg/ml)     
    IL-6 6.54 ± 0.30 8.90 ± 0.84* 6.79 ± 0.53 1.98 ± 0.19 
    IL-8 847 ± 32.45 16,683 ± 4,474* 1,791 ± 145 325 ± 3.51 
    MCP-1 0.10 ± 0.01 2.33 ± 0.01* 0.28 ± 0.26 0.10 ± 0.01 
    MIP-1β 4.50 ± 0.12 12.66 ± 0.58* 4.26 ± 0.21 0.775 ± 0.08 
NHP/2 (pg/ml)     
    IL-6 62.21 ± 3.75 216 ± 45* ND 57.36 ± 9.40 
    IL-8 2.01 ± 0.01 6,841 ± 764* ND 1,842 ± 272 
    MCP-1 1,325 ± 56 2,897 ± 448* ND 616 ± 145 
    MIP-1β 19 ± 0.90 84 ± 11.55* ND 8.90 ± 1.27 
NHP/3 (pg/ml)     
    IL-6 0.10 ± 0.01 1.29 ± 0.21* 1.29 ± 1.29 ND 
    IL-8 162 ± 3.67 567 ± 51.9* 246 ± 82.39 ND 
    MCP-1 103 ± 6.09 356 ± 3.13* 161 ± 43.55 ND 
    MIP-1β 4.04 ± 0.11 17 ± 0.81* 6.39 ± 2.05 ND 
ControlCD40LPD98059 + CD40LBay 117082 + CD40L
NHP/1 (pg/ml)     
    IL-6 4.12 ± 2.61 10.18 ± 1.09* 5.10 ± 0.32 2.69 ± 0.37 
    IL-8 635 ± 248 1,701 ± 396* 463 ± 7.69 55 ± 1.88 
    MCP-1 181 ± 43 361 ± 57* 159 ± 8.30 17.39 ± 0.10 
    MIP-1β 2.55 ± 0.55 31.60 ± 2.59* 10.62 ± 0.24 1.20 ± 0.09 
Human islets (pg/ml)     
    IL-6 6.54 ± 0.30 8.90 ± 0.84* 6.79 ± 0.53 1.98 ± 0.19 
    IL-8 847 ± 32.45 16,683 ± 4,474* 1,791 ± 145 325 ± 3.51 
    MCP-1 0.10 ± 0.01 2.33 ± 0.01* 0.28 ± 0.26 0.10 ± 0.01 
    MIP-1β 4.50 ± 0.12 12.66 ± 0.58* 4.26 ± 0.21 0.775 ± 0.08 
NHP/2 (pg/ml)     
    IL-6 62.21 ± 3.75 216 ± 45* ND 57.36 ± 9.40 
    IL-8 2.01 ± 0.01 6,841 ± 764* ND 1,842 ± 272 
    MCP-1 1,325 ± 56 2,897 ± 448* ND 616 ± 145 
    MIP-1β 19 ± 0.90 84 ± 11.55* ND 8.90 ± 1.27 
NHP/3 (pg/ml)     
    IL-6 0.10 ± 0.01 1.29 ± 0.21* 1.29 ± 1.29 ND 
    IL-8 162 ± 3.67 567 ± 51.9* 246 ± 82.39 ND 
    MCP-1 103 ± 6.09 356 ± 3.13* 161 ± 43.55 ND 
    MIP-1β 4.04 ± 0.11 17 ± 0.81* 6.39 ± 2.05 ND 

Data are means ± SD. NHP/1, 2, and 3 represent three different islet preparations, respectively.

*

P ≤ 0.05 vs. control,

P ≤ 0.05 vs. PD + CD40L,

P ≤ 0.05 vs. Bay + CD40L. ND, not determined.

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