Primers used for PCR analysis
| Gene . | Sense primer . | Antisense primer . | Product size (bp) . |
|---|---|---|---|
| HPRT | gctggtgaaaaggacctct | cacaggactagaacacctgc | 249 |
| E-cad | gcagtcagatctccctgagttcgag | gttgctagagtgacctttgtatgtag | 372 |
| Pdx1 | ctatccttcaacctataccatttc | gaaatcagccaggttgccttcaac | 409 |
| Ptf1a | catagagaacgaaccaccctttgag | gcacggagtttcctggacagagttc | 294 |
| Hnf6 | gcaatggaagtaattcagggcag | catgaagaagttgctgacagtgc | 471 |
| Hnf4α | ctcttctgattataagctgaggatg | ccacaggaaggtgcagattgatctg | 377 |
| Foxa2 | cctctatgtagactactgcttctc | cctggatttcaccatgtccagaatg | 277 |
| Hnf1β | gttgaaattccaagagtgacttgctc | ctttaatgggaggcttcctgagatg | 281 |
| Hlxb9 | caagctcaacaagtacctgtctcg | gcaccattgctgtacgggaagttg | 341 |
| NeuroD | cttggccaagaactacatctgg | ggagtagggatgcaccgggaa | 222 |
| Ngn3 | ggtagcactacctagttggagactc | gacaaacagtgcttcaggaaccgtc | 389 |
| Nkx2.2 | ctaaatatttatggccatgtacacg | gttccaagctccgatgctcaggag | 325 |
| Insulin1 | ccagctataatcagagacca | gtgtagaagaagccacgct | 197 |
| Glucagon | actcacagggcacattcacc | ccagttgatgaagtccctgg | 353 |
| Gene . | Sense primer . | Antisense primer . | Product size (bp) . |
|---|---|---|---|
| HPRT | gctggtgaaaaggacctct | cacaggactagaacacctgc | 249 |
| E-cad | gcagtcagatctccctgagttcgag | gttgctagagtgacctttgtatgtag | 372 |
| Pdx1 | ctatccttcaacctataccatttc | gaaatcagccaggttgccttcaac | 409 |
| Ptf1a | catagagaacgaaccaccctttgag | gcacggagtttcctggacagagttc | 294 |
| Hnf6 | gcaatggaagtaattcagggcag | catgaagaagttgctgacagtgc | 471 |
| Hnf4α | ctcttctgattataagctgaggatg | ccacaggaaggtgcagattgatctg | 377 |
| Foxa2 | cctctatgtagactactgcttctc | cctggatttcaccatgtccagaatg | 277 |
| Hnf1β | gttgaaattccaagagtgacttgctc | ctttaatgggaggcttcctgagatg | 281 |
| Hlxb9 | caagctcaacaagtacctgtctcg | gcaccattgctgtacgggaagttg | 341 |
| NeuroD | cttggccaagaactacatctgg | ggagtagggatgcaccgggaa | 222 |
| Ngn3 | ggtagcactacctagttggagactc | gacaaacagtgcttcaggaaccgtc | 389 |
| Nkx2.2 | ctaaatatttatggccatgtacacg | gttccaagctccgatgctcaggag | 325 |
| Insulin1 | ccagctataatcagagacca | gtgtagaagaagccacgct | 197 |
| Glucagon | actcacagggcacattcacc | ccagttgatgaagtccctgg | 353 |