Skip to Main Content
TABLE 1

Metabolic and blood pressure data in WT and iNOS knockout mice fed a standard or high-fat diet at 4 and 8 weeks of feeding

4 weeks8 weeks
Body mass (g)   
    WT, standard 24.1 ± 0.3 29.1 ± 0.4 
    WT, high-fat 27.3 ± 0.4* 38.4 ± 0.4* 
    iNOS KO, standard 22.0 ± 0.8 27.2 ± 1.1 
    iNOS KO, high-fat 27.9 ± 0.5 36.6 ± 1.3 
Epididymal fat pad (mg)   
    WT, standard 11.7 ± 1.1 12.0 ± 0.3 
    WT, high-fat 24.4 ± 1.5* 33.6 ± 0.9* 
    iNOS KO, standard 11.3 ± 0.5 13.2 ± 0.5 
    iNOS KO, high-fat 23.4 ± 0.8 32.1 ± 0.7 
Fasting glucose (mmol/l)   
    WT, standard 6.4 ± 0.6 6.5 ± 0.4 
    WT, high-fat 12.0 ± 0.3* 11.2 ± 0.6* 
    iNOS KO, standard 7.0 ± 0.2 6.1 ± 0.2 
    iNOS KO, high-fat 8.5 ± 0.5 7.7 ± 0.2 
Fasting insulin (pg/ml)   
    WT, standard 0.5 ± 0.1 0.3 ± 0.1 
    WT, high-fat 1.0 ± 0.2* 3.5 ± 0.5* 
    iNOS KO, standard 0.5 ± 0.1 0.4 ± 0.1 
    iNOS KO, high-fat 0.6 ± 0.1 0.5 ± 0.1 
HOMA (unit)   
    WT, standard 0.15 ± 0.01 0.15 ± 0.2 
    WT, high-fat 0.55 ± 0.1* 2.03 ± 0.2* 
    iNOS KO, standard 0.14 ± 0.01 0.13 ± 0.01 
    iNOS KO, high-fat 0.18 ± 0.01 0.22 ± 0.02 
Free fatty acids (mmol/l)   
    WT, standard — 0.42 ± 0.1 
    WT, high-fat — 1.41 ± 0.1* 
    iNOS KO, standard — 0.35 ± 0.1 
    iNOS KO, high-fat — 0.56 ± 0.1 
Decline in glucose in response to insulin (%)   
    WT, standard 70.2 ± 1.9 65.6 ± 1.2 
    WT, high-fat 54.9 ± 0.6* 48.5 ± 1.7* 
    iNOS KO, standard 68.1 ± 2.6 66.2 ± 1.7 
    iNOS KO, high-fat 68.0 ± 1.3 68.5 ± 1.2 
Systolic blood pressure (mmHg)   
    WT, standard 117.6 ± 0.3 118.6 ± 0.5 
    WT, high-fat 122.7 ± 0.3* 124.8 ± 0.7* 
    iNOS KO, standard 113.9 ± 0.4 114.9 ± 0.5 
    iNOS KO, high-fat 122.5 ± 0.5 124.0 ± 0.7 
4 weeks8 weeks
Body mass (g)   
    WT, standard 24.1 ± 0.3 29.1 ± 0.4 
    WT, high-fat 27.3 ± 0.4* 38.4 ± 0.4* 
    iNOS KO, standard 22.0 ± 0.8 27.2 ± 1.1 
    iNOS KO, high-fat 27.9 ± 0.5 36.6 ± 1.3 
Epididymal fat pad (mg)   
    WT, standard 11.7 ± 1.1 12.0 ± 0.3 
    WT, high-fat 24.4 ± 1.5* 33.6 ± 0.9* 
    iNOS KO, standard 11.3 ± 0.5 13.2 ± 0.5 
    iNOS KO, high-fat 23.4 ± 0.8 32.1 ± 0.7 
Fasting glucose (mmol/l)   
    WT, standard 6.4 ± 0.6 6.5 ± 0.4 
    WT, high-fat 12.0 ± 0.3* 11.2 ± 0.6* 
    iNOS KO, standard 7.0 ± 0.2 6.1 ± 0.2 
    iNOS KO, high-fat 8.5 ± 0.5 7.7 ± 0.2 
Fasting insulin (pg/ml)   
    WT, standard 0.5 ± 0.1 0.3 ± 0.1 
    WT, high-fat 1.0 ± 0.2* 3.5 ± 0.5* 
    iNOS KO, standard 0.5 ± 0.1 0.4 ± 0.1 
    iNOS KO, high-fat 0.6 ± 0.1 0.5 ± 0.1 
HOMA (unit)   
    WT, standard 0.15 ± 0.01 0.15 ± 0.2 
    WT, high-fat 0.55 ± 0.1* 2.03 ± 0.2* 
    iNOS KO, standard 0.14 ± 0.01 0.13 ± 0.01 
    iNOS KO, high-fat 0.18 ± 0.01 0.22 ± 0.02 
Free fatty acids (mmol/l)   
    WT, standard — 0.42 ± 0.1 
    WT, high-fat — 1.41 ± 0.1* 
    iNOS KO, standard — 0.35 ± 0.1 
    iNOS KO, high-fat — 0.56 ± 0.1 
Decline in glucose in response to insulin (%)   
    WT, standard 70.2 ± 1.9 65.6 ± 1.2 
    WT, high-fat 54.9 ± 0.6* 48.5 ± 1.7* 
    iNOS KO, standard 68.1 ± 2.6 66.2 ± 1.7 
    iNOS KO, high-fat 68.0 ± 1.3 68.5 ± 1.2 
Systolic blood pressure (mmHg)   
    WT, standard 117.6 ± 0.3 118.6 ± 0.5 
    WT, high-fat 122.7 ± 0.3* 124.8 ± 0.7* 
    iNOS KO, standard 113.9 ± 0.4 114.9 ± 0.5 
    iNOS KO, high-fat 122.5 ± 0.5 124.0 ± 0.7 

Data are means ± SE.

*

P < 0.05 for standard vs. high-fat diet;

P < 0.05 for WT vs. iNOS KO mice on a high-fat diet.

Close Modal

or Create an Account

Close Modal
Close Modal