Frequency of induced diabetes in LEW0.1WR1 rats
| Treatment type . | Group . | Poly I:C . | Virus . | n . | n (%) diabetic . |
|---|---|---|---|---|---|
| Culture medium alone | 1 | No | None | 16 | 0 (0) |
| Single viral inoculations* | 2 | No | KRV alone | 8 | 3 (38) |
| 3 | No | RCMV alone†‡ | 38 | 14 (37) | |
| 4 | No | Coxsackie B4 alone§ | 18 | 0 (0) | |
| 5 | No | Vaccinia alone‖ | 14 | 1 (7) | |
| 6 | No | H-1 alone | 20 | 0 (0) | |
| 7 | Yes | None | 18 | 0 (0) | |
| 8 | No | RCMV + KRV | 45 | 34 (76) | |
| Simultaneous viral inoculations¶ | 9 | No | RCMV + CoxB4 | 8 | 1 (13) |
| 10 | Yes | KRV | 6 | 6 (100) | |
| Sequential treatments** | 11 | Yes | RCMV | 29 | 3 (10) |
| 12 | Yes | Coxsackie B4 | 18 | 3 (17) | |
| 13 | Yes | Vaccinia | 10 | 4 (40) | |
| 14 | Yes | H-1 | 6 | 0 (0) |
| Treatment type . | Group . | Poly I:C . | Virus . | n . | n (%) diabetic . |
|---|---|---|---|---|---|
| Culture medium alone | 1 | No | None | 16 | 0 (0) |
| Single viral inoculations* | 2 | No | KRV alone | 8 | 3 (38) |
| 3 | No | RCMV alone†‡ | 38 | 14 (37) | |
| 4 | No | Coxsackie B4 alone§ | 18 | 0 (0) | |
| 5 | No | Vaccinia alone‖ | 14 | 1 (7) | |
| 6 | No | H-1 alone | 20 | 0 (0) | |
| 7 | Yes | None | 18 | 0 (0) | |
| 8 | No | RCMV + KRV | 45 | 34 (76) | |
| Simultaneous viral inoculations¶ | 9 | No | RCMV + CoxB4 | 8 | 1 (13) |
| 10 | Yes | KRV | 6 | 6 (100) | |
| Sequential treatments** | 11 | Yes | RCMV | 29 | 3 (10) |
| 12 | Yes | Coxsackie B4 | 18 | 3 (17) | |
| 13 | Yes | Vaccinia | 10 | 4 (40) | |
| 14 | Yes | H-1 | 6 | 0 (0) |
Twenty- to 25-day-old male and female LEW0.1WR1 rats were inoculated intraperitoneally with the indicated viral agents with or without pretreatment with poly I:C as described in research design and methods. Doses were as follows: KRV, 107 PFU; H-1, 107 PFU; CoxB4, 108 PFU; VV, 106 PFU; or RCMV, 104 PFU.
*Overall χ2 for single virus inoculation category = 25.48, df = 5, P = 0.001.
†Group 3 vs. group 8, Fisher exact statistic, P = 0.0007.
‡Group 3 vs. group 11: Fisher exact statistic, P = 0.02.
§Group 4 vs. group 12, P = NS.
‖Group 5 vs. group 13, Fisher exact statistic, P = NS.
¶Group 8 vs. group 9, Fisher exact statistic, P = 0.001.
**Overall χ2 for sequential treatments category = 25.58, df = 3, P < 0.001. In all experiments in which diabetes occurred, both male and female rats were affected. In groups 3 and 8 in which there were larger numbers of diabetic animals, both sexes were affected in approximately equal proportions.