Genes regulated by high glucose or high glucose plus FOXO1 siRNA in RMECs
| Gene . | Unique identification . | High glucose . | High glucose plus FOXO1 siRNA . | ||
|---|---|---|---|---|---|
| Mean . | Median . | Mean . | Median . | ||
| Endothelial cell inflammation/activation | |||||
| CCL2 | Rn.4772 | 2.2 | 1.7 | 0.4 | 0.3 |
| CCL5 | Rn.8019 | 2.1 | 2.1 | 0.1 | 0.1 |
| CXCL1 | Rn.10907 | 2.8 | 2.5 | 0.3 | 0.2 |
| ICAM1 | Rn0.12 | 2.3 | 2.0 | 0.4 | 0.4 |
| IL6 | Rn.9873 | 2.4 | 2.0 | 0.3 | 0.3 |
| IL7 | Rn.10793 | 2.3 | 1.7 | 0.3 | 0.2 |
| VCAM1 | Rn.11267 | 2.8 | 2.0 | 0.3 | 0.3 |
| ANXA5 | Rn.3318 | 1.6 | 1.6 | 0.6 | 0.6 |
| CCL20 | Rn.10722 | 2.4 | 1.6 | 0.3 | 0.3 |
| CPB2 | Rn.12572 | 1.9 | 1.2 | 0.5 | 0.6 |
| CSF2 | Rn.44285 | 1.4 | 1.2 | 0.3 | 0.2 |
| ICAM2 | Rn.138519 | 3.0 | 1.1 | 0.2 | 0.2 |
| INOS | Rn.10400 | 1.9 | 1.3 | 0.4 | 0.5 |
| SEL-E | Rn.10359 | 1.7 | 1.0 | 0.4 | 0.3 |
| SEL-L | Rn.10461 | 1.8 | 1.1 | 0.3 | 0.3 |
| SEL-P | Rn.10012 | 2.0 | 1.2 | 0.3 | 0.3 |
| TGFB1 | Rn.40136 | 2.6 | 1.5 | 0.3 | 0.2 |
| TGFB2 | Rn.24539 | 2.3 | 1.5 | 0.3 | 0.2 |
| VWF | Rn.35561 | 1.7 | 1.1 | 0.4 | 0.3 |
| IL11 | Rn.47218 | 1.4 | 0.7 | 0.3 | 0.5 |
| IL15 | Rn.2490 | 2.0 | 1.1 | 0.2 | 0.2 |
| IL3 | Rn.10652 | 1.0 | 0.5 | 0.3 | 0.6 |
| Endothelial cell apoptosis | |||||
| BAX | Rn.10668 | 2.5 | 2.0 | 0.5 | 0.5 |
| BCL2 | Rn.9996 | 2.5 | 2.1 | 0.4 | 0.4 |
| CASP3 | Rn.10562 | 2.9 | 2.4 | 0.5 | 0.5 |
| CASP6 | Rn.88160 | 4.1 | 4.2 | 0.3 | 0.3 |
| CASP8 | Rn.54474 | 2.0 | 1.9 | 0.5 | 0.5 |
| TNFRSF11B | Rn.9792 | 1.7 | 1.7 | 0.3 | 0.4 |
| TNFRSF10B | Rn.105558 | 2.6 | 1.5 | 0.3 | 0.4 |
| BCL2A1 | Rn.19770 | 1.7 | 1.7 | 0.7 | 0.7 |
| BIRC3 | Rn.64578 | 1.8 | 1.7 | 0.7 | 0.7 |
| BIRC4 | Rn.91239 | 2.8 | 1.7 | 0.7 | 0.8 |
| Angiogenesis | |||||
| ITGB1 | Rn.25733 | 2.6 | 2.0 | 0.3 | 0.3 |
| ANGPT1 | Rn.120272 | 4.9 | 4.6 | 0.4 | 0.4 |
| FLT4 | Rn.81043 | 3.5 | 2.7 | 0.2 | 0.2 |
| VEGFA | Rn.1923 | 3.0 | 2.8 | 0.4 | 0.3 |
| ITGA5 | Rn.100796 | 2.8 | 1.1 | 0.4 | 0.4 |
| ITGB3 | Rn.17129 | 2.4 | 1.4 | 0.3 | 0.3 |
| KDR | Rn.88869 | 1.9 | 1.1 | 0.4 | 0.4 |
| MMP2 | Rn.6422 | 2.5 | 1.6 | 0.4 | 0.4 |
| MMP9 | Rn.10209 | 2.1 | 1.3 | 0.3 | 0.4 |
| TEK | Rn.9159 | 3.9 | 1.6 | 0.3 | 0.3 |
| COL18A1 | Rn.12030 | 2.2 | 1.3 | 0.5 | 0.5 |
| PLG | Rn.20178 | 1.7 | 1.1 | 0.5 | 0.6 |
| Gene . | Unique identification . | High glucose . | High glucose plus FOXO1 siRNA . | ||
|---|---|---|---|---|---|
| Mean . | Median . | Mean . | Median . | ||
| Endothelial cell inflammation/activation | |||||
| CCL2 | Rn.4772 | 2.2 | 1.7 | 0.4 | 0.3 |
| CCL5 | Rn.8019 | 2.1 | 2.1 | 0.1 | 0.1 |
| CXCL1 | Rn.10907 | 2.8 | 2.5 | 0.3 | 0.2 |
| ICAM1 | Rn0.12 | 2.3 | 2.0 | 0.4 | 0.4 |
| IL6 | Rn.9873 | 2.4 | 2.0 | 0.3 | 0.3 |
| IL7 | Rn.10793 | 2.3 | 1.7 | 0.3 | 0.2 |
| VCAM1 | Rn.11267 | 2.8 | 2.0 | 0.3 | 0.3 |
| ANXA5 | Rn.3318 | 1.6 | 1.6 | 0.6 | 0.6 |
| CCL20 | Rn.10722 | 2.4 | 1.6 | 0.3 | 0.3 |
| CPB2 | Rn.12572 | 1.9 | 1.2 | 0.5 | 0.6 |
| CSF2 | Rn.44285 | 1.4 | 1.2 | 0.3 | 0.2 |
| ICAM2 | Rn.138519 | 3.0 | 1.1 | 0.2 | 0.2 |
| INOS | Rn.10400 | 1.9 | 1.3 | 0.4 | 0.5 |
| SEL-E | Rn.10359 | 1.7 | 1.0 | 0.4 | 0.3 |
| SEL-L | Rn.10461 | 1.8 | 1.1 | 0.3 | 0.3 |
| SEL-P | Rn.10012 | 2.0 | 1.2 | 0.3 | 0.3 |
| TGFB1 | Rn.40136 | 2.6 | 1.5 | 0.3 | 0.2 |
| TGFB2 | Rn.24539 | 2.3 | 1.5 | 0.3 | 0.2 |
| VWF | Rn.35561 | 1.7 | 1.1 | 0.4 | 0.3 |
| IL11 | Rn.47218 | 1.4 | 0.7 | 0.3 | 0.5 |
| IL15 | Rn.2490 | 2.0 | 1.1 | 0.2 | 0.2 |
| IL3 | Rn.10652 | 1.0 | 0.5 | 0.3 | 0.6 |
| Endothelial cell apoptosis | |||||
| BAX | Rn.10668 | 2.5 | 2.0 | 0.5 | 0.5 |
| BCL2 | Rn.9996 | 2.5 | 2.1 | 0.4 | 0.4 |
| CASP3 | Rn.10562 | 2.9 | 2.4 | 0.5 | 0.5 |
| CASP6 | Rn.88160 | 4.1 | 4.2 | 0.3 | 0.3 |
| CASP8 | Rn.54474 | 2.0 | 1.9 | 0.5 | 0.5 |
| TNFRSF11B | Rn.9792 | 1.7 | 1.7 | 0.3 | 0.4 |
| TNFRSF10B | Rn.105558 | 2.6 | 1.5 | 0.3 | 0.4 |
| BCL2A1 | Rn.19770 | 1.7 | 1.7 | 0.7 | 0.7 |
| BIRC3 | Rn.64578 | 1.8 | 1.7 | 0.7 | 0.7 |
| BIRC4 | Rn.91239 | 2.8 | 1.7 | 0.7 | 0.8 |
| Angiogenesis | |||||
| ITGB1 | Rn.25733 | 2.6 | 2.0 | 0.3 | 0.3 |
| ANGPT1 | Rn.120272 | 4.9 | 4.6 | 0.4 | 0.4 |
| FLT4 | Rn.81043 | 3.5 | 2.7 | 0.2 | 0.2 |
| VEGFA | Rn.1923 | 3.0 | 2.8 | 0.4 | 0.3 |
| ITGA5 | Rn.100796 | 2.8 | 1.1 | 0.4 | 0.4 |
| ITGB3 | Rn.17129 | 2.4 | 1.4 | 0.3 | 0.3 |
| KDR | Rn.88869 | 1.9 | 1.1 | 0.4 | 0.4 |
| MMP2 | Rn.6422 | 2.5 | 1.6 | 0.4 | 0.4 |
| MMP9 | Rn.10209 | 2.1 | 1.3 | 0.3 | 0.4 |
| TEK | Rn.9159 | 3.9 | 1.6 | 0.3 | 0.3 |
| COL18A1 | Rn.12030 | 2.2 | 1.3 | 0.5 | 0.5 |
| PLG | Rn.20178 | 1.7 | 1.1 | 0.5 | 0.6 |
Data are mean and median of three independent arrays for each time point. Boldface indicates that both the mean and median of the experimental group was 1.7- or 0.58-fold of the corresponding control (high glucose versus low glucose; high glucose plus FOXO1 siRNA versus high glucose plus scrambled siRNA). RMECs were incubated with or without high glucose (25 mmol/l) for 5 days. Some cells were transfected with FOXO1 siRNA or scrambled siRNA for 24 h prior to incubation in high glucose for 5 days. Total RNA was isolated and subjected to focused microarray analysis. The table lists genes differentially regulated by high glucose or FOXO1 siRNA in high-glucose–treated microvascular endothelial cells. High glucose: fold change in mRNA level in high-glucose–treated cells versus standard-glucose–treated cells. High glucose plus FOXO1 siRNA: fold change in mRNA level of each gene in cells transfected with FOXO1 siRNA plus high glucose versus scrambled siRNA plus high glucose.