IL-6RαΔβ mice are more susceptible to alloxan-induced β-cell oxidative stress. A: Design of experiments applying alloxan treatment. B: Blood glucose levels in mice fed randomly were measured daily for 5 days after alloxan injection. C: Terminal serum insulin levels from samples collected 6 h after injection. D: Representative images of pancreata collected either 6 h or 8 days after alloxan treatment (left), from which β-cell mass was calculated; insulin staining appears brown. Quantification of β-cell mass is shown in the graph to the right. E: Representative images of pancreata collected 6 h after injection and immunostained for insulin (Ins; green) and 4-HNE (red); DAPI-stained nuclei appear blue (left). Quantification of islet 4-HNE is shown in the graph to the right. Data are mean ± SEM for 3–6 individual mice. *P < 0.05. KO, knockout; ns, not significant; WT, wild type.
